Related Experiment Video
Updated: Jun 3, 2026
11:12
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
8.0K
Identification of Tumor Budding-Associated Genes in Breast Cancer through Transcriptomic Profiling and Network
Panisa Janyasupab1, Kodchanan Singhanat2, Malee Warnnissorn3
1Advance Virtual and Intelligent Computing (AVIC) Center, Department of Mathematics and Computer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Biomolecules
|August 29, 2024
Summary
Researchers identified seven novel genes linked to high tumor budding in breast cancer. These genes and associated signaling pathways may offer new therapeutic targets for breast cancer treatment and diagnosis.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Breast cancer is the most diagnosed cancer globally.
- Tumor budding (TB) is an emerging prognostic marker for breast cancer.
- Identifying genes associated with high TB is critical for understanding tumor progression and metastasis.
Purpose of the Study:
- To identify novel gene biomarkers specific to high tumor budding (TB) breast cancer samples.
- To explore the functional roles of these identified genes in cancer progression.
- To discover potential therapeutic targets for breast cancer.
Main Methods:
- TempO-Seq RNA sequencing was used to profile transcriptomic data from breast cancer samples.
- Differential gene expression analysis and mutual information were employed to identify candidate biomarkers.
- Protein-protein interaction network analysis and network diffusion were utilized to explore pathway enrichment.
Main Results:
- Seven genes (NOL4, STAR, C8G, NEIL1, SLC46A3, FRMD6, SCARF2) were identified as potential biomarkers for high-TB breast cancer.
- These genes are overexpressed in high-TB samples.
- Enrichment analysis revealed significant involvement of the Hippo and Wnt signaling pathways.
Conclusions:
- The identified genes and their associated pathways (Hippo, Wnt) are promising therapeutic targets for breast cancer.
- These findings advance the understanding of breast cancer progression and metastasis.
- This research offers potential for improved diagnosis and treatment strategies for breast cancer.
Related Concept Videos
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...