Related Experiment Video
Updated: Jun 14, 2025
10:51
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
12.7K
Cladosporols and PPARγ: Same Gun, Same Bullet, More Targets
Roberta Rapuano1, Antonella Mercuri1, Sabrina Dallavalle2
1Dipartimento di Scienze e Tecnologie, Università del Sannio, Via dei Mulini, 82100 Benevento, Italy.
Biomolecules
|August 29, 2024
Summary
Cladosporols, derived from the fungus Cladosporium tenuissimum, activate PPARγ. This interaction shows potential for treating metabolic disorders and cancer by regulating lipid metabolism and cell proliferation.
Area of Science:
- Natural product chemistry
- Molecular biology
- Pharmacology
Background:
- Peroxisome proliferator-activated receptor gamma (PPARγ) agonists regulate key biological processes.
- Dysregulated lipid metabolism and cancer are significant health concerns.
Purpose of the Study:
- To explore the molecular mechanisms of Cladosporols as PPARγ modulators.
- To highlight the therapeutic potential of Cladosporols in metabolic diseases and cancer.
Main Methods:
- Purification of Cladosporols from Cladosporium tenuissimum.
- Assays to evaluate PPARγ agonistic activity.
- In vitro studies on cancer cell lines and preadipocytes.
Main Results:
- Cladosporols effectively control cell proliferation in colorectal and prostate cancer cells via PPARγ.
- Cladosporols demonstrate strong anti-adipogenetic activity, inhibiting adipocyte differentiation.
- The Cladosporol/PPARγ complex influences lipid metabolism and cancer progression.
Conclusions:
- Cladosporols exhibit dual therapeutic potential for metabolic disorders and cancer.
- Targeting the Cladosporol/PPARγ interaction may offer novel treatment strategies.
- Further research is warranted to explore Cladosporols' benefits for human health.
Related Concept Videos
01:31Transducer Mechanism: Nuclear Receptors
1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
01:19Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
01:30Assembly of Signaling Complexes
5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
01:26Signal Transduction: Overview
8.3K
Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
Typically, signal transduction involves three...
8.3K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K