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A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis
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Cardiovascular and Renal Effects Induced by Alpha-Lipoic Acid Treatment in Two-Kidney-One-Clip Hypertensive Rats
Déborah Victória Gomes Nascimento1, Darlyson Ferreira Alencar2, Matheus Vinicius Barbosa da Silva1
1Laboratory of Nutrition, Physical Activity and Phenotypic Plasticity, Federal University of Pernambuco-UFPE, Vitória de Santo Antão 55608-680, Brazil.
Biomedicines
|August 29, 2024
Summary
Alpha-lipoic acid (LA) treatment in hypertensive rats did not reverse cardiac or kidney changes. However, LA mitigated the increased blood vessel contraction associated with hypertension.
Area of Science:
- Cardiovascular Research
- Nephrology
- Oxidative Stress Biology
Background:
- Hypertension is linked to oxidative stress, leading to kidney injury and endothelial dysfunction.
- Alpha-lipoic acid (LA) is a potent antioxidant with potential therapeutic benefits.
Purpose of the Study:
- To investigate the cardiovascular and renal effects of alpha-lipoic acid (LA) in a rat model of two-kidney-one-clip (2K1C) induced hypertension.
- To assess LA's impact on vascular reactivity, cardiac mass, and kidney function in hypertension.
Main Methods:
- Utilized the two-kidney-one-clip (2K1C) surgical model in rats to induce hypertension.
- Administered alpha-lipoic acid (LA) to hypertensive and sham-operated rats for 14 days.
- Evaluated food/water intake, urinary volume, kidney weight ratio, cardiac mass, vascular reactivity (phenylephrine, acetylcholine, sodium nitroprusside), and serum urea/creatinine levels.
Main Results:
- Hypertension (2K1C) significantly increased the left/right kidney weight ratio.
- LA treatment did not alter food/water intake, urinary volume, cardiac mass, or kidney/urea/creatinine levels.
- LA treatment increased phenylephrine-induced contraction potency in hypertensive rats but did not improve vasorelaxation to acetylcholine or sodium nitroprusside.
Conclusions:
- Alpha-lipoic acid (LA) did not reverse the morphological cardiac or renal changes induced by 2K1C hypertension.
- LA treatment mitigated the enhanced vascular contraction observed in 2K1C hypertensive rats.
- Further research is needed to elucidate the precise mechanisms of LA's vascular effects in hypertension.
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