Related Experiment Video
Updated: Jun 14, 2025
14:18
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
20.9K
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Applications for Metabolomics
Darcy Cochran1,2, Robert Powers1,2
1Department of Chemistry, University of Nebraska-Lincoln, 722 Hamilton Hall, Lincoln, NE 68588-0304, USA.
Biomedicines
|August 29, 2024
Summary
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) offers high resolution and sensitivity for metabolomics. This review highlights its applications and practical considerations for researchers seeking detailed metabolome characterization.
Area of Science:
- Metabolomics
- Analytical Chemistry
- Biochemistry
Background:
- Metabolomics studies all metabolites (< 1500 Da) in organisms, rapidly growing with high-resolution mass spectrometry (HRMS).
- Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is an HRMS technique with exceptional resolution (10^5-10^6) and mass accuracy (ppb).
Purpose of the Study:
- To review recent applications of FT-ICR-MS in metabolomics research.
- To highlight the advantages of FT-ICR-MS for detailed and reliable metabolome characterization.
- To provide practical considerations for implementing FT-ICR-MS in research programs.
Main Methods:
- Review of recent metabolomics studies utilizing FT-ICR-MS.
- Examination of FT-ICR-MS capabilities including separation of isobars/isomers, isotopic fine structure analysis, and spatial resolution.
- Discussion of data analysis methods (e.g., van Krevelen diagrams, Kendrick mass defect plots).
Main Results:
- FT-ICR-MS enables high-confidence metabolite identification (<1 ppm mass error) and analysis of complex biological systems.
- Applications include biomarker discovery (clinical and non-human), food/beverage/environmental analysis, and high-resolution tissue imaging.
- Advantages include easy separation of isobaric/isomeric species and isotopic fine structure analysis.
Conclusions:
- FT-ICR-MS is a powerful tool for advancing metabolomics due to its high resolution, sensitivity, and unique analytical capabilities.
- Despite challenges like cost and data complexity, FT-ICR-MS provides invaluable insights for detailed metabolome characterization.
- This review offers practical guidance and resources for researchers interested in adopting FT-ICR-MS for metabolomics studies.
More Related Videos
Related Concept Videos
01:16Mass Spectrometers
5.3K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.3K
01:19Mass Spectrometry: Overview
4.7K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
4.7K
01:21Mass Spectrometry: Complex Analysis
736
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
736
01:15High-Resolution Mass Spectrometry (HRMS)
1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
01:19Mass Analyzers: Common Types
579
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
579
01:01Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
1.2K
The fragmentation patterns observed for compounds such as carboxylic acids, esters, and amides in the mass spectra include ⍺-cleavage and McLafferty rearrangement. Fragmentation by ⍺-cleavage preferentially occurs at the carbon-carbon bond at the ⍺-position next to the carboxylic group to generate a neutral radical and a cation. Long chain compounds with hydrogen at their γ-carbon undergo McLafferty rearrangement to give a radical cation and a neutral alkene.
For example,...
For example,...
1.2K