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Updated: Jun 24, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Functional Cyperus esculentus L. Cookies Enriched with the Probiotic Strain Lacticaseibacillus rhamnosus SL42
Noussaiba Belmadani1, Wafa Kassous1, Kawtar Keddar1
1ProbiotSanté Team, Laboratoire des Micro-Organismes Bénéfiques, des Aliments Fonctionnels et de la Santé (LMBAFS), Abdelhamid Ibn Badis University, Hocine Hamadou Street, Mostaganem 27000, Algeria.
Diabetic-friendly cookies made with organic tiger nut flour offer antioxidant benefits. Enriched cookies initially rated higher but control versions were preferred long-term, with the probiotic surviving storage.
Area of Science:
- Food Science
- Nutritional Science
- Microbiology
Background:
- Developing functional foods for diabetic management is crucial.
- Tiger nut (Cyperus esculentus L.) flour offers potential due to its nutritional profile.
- Incorporating probiotics into food matrices presents challenges for viability and stability.
Purpose of the Study:
- To create and evaluate functional cookies for diabetics using organic tiger nut flour.
- To assess the impact of polyfloral honey syrup with Lacticaseibacillus rhamnosus SL42 on cookie properties.
- To determine the antioxidant, antibacterial, and sensory characteristics of the developed cookies.
Main Methods:
- Chemical, rheological, and functional analyses of tiger nut flour.
- Sensory evaluation by 33 diabetic and semi-naive panelists.
- Microbiological (MRS-cys agar) and SEM analyses for probiotic survival and adhesion.
- CIE LAB color analysis and physical property assessment.
Main Results:
- Tiger nut flour exhibits rich polyphenol and flavonoid content with antioxidant and antibacterial properties.
- Both cookie types met international standards; honey-enriched cookies initially scored higher sensorially, but control cookies were preferred after 15 days.
- Lacticaseibacillus rhamnosus SL42 remained viable (6.43 Log CFU/cookie) and adhered to the surface of honey-enriched cookies after 21 days.
Conclusions:
- Functional cookies for diabetics can be successfully produced using organic tiger nut flour.
- Honey syrup enrichment supports probiotic viability and adhesion, though long-term sensory preference favors control cookies.
- Further research is needed to explore the therapeutic potential of these functional cookies.
Related Concept Videos
Microbes in Food Production
Microbes in the Production of Fermented Foods
Probiotics
Bioreactor Controls-III
Production of Organic Acids
Microbiota of the Large Intestine

