Adenosine 5'-triphosphate stimulates the release of polypeptides from mitochondria

There was release of polypeptides to the medium when mitochondria containing labeled proteins were incubated with a rat liver post-mitochondrial supernatant. The release of polypeptides increased with the amount of rat liver extract added. Addition of cycloheximide did not inhibit the effect. Heating the post-mitochondrial supernatant did not inhibit the release of mitochondrial proteins, indicating that it was due to a heat-stable factor. The factor responsible has been isolated and identified as ATP. The presence of EDTA inhibits the release of polypeptides caused by ATP and Mg2+ stimulates it. The possible role of ATP in the turnover of mitochondrial proteins is briefly discussed.