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Integrated Transcriptomic-Metabolomic Analysis Reveals the Effect of Different Light Intensities on Ovarian
Xiaoli Zhou1,2, Yuhang Xu1,2, Cheng Fang1,2
1College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
International Journal of Molecular Sciences
|August 29, 2024
Summary
Low light intensity delays egg laying in partridge breeders by altering ovarian development. This study reveals key genes and metabolites involved in light
Area of Science:
- Animal Science
- Reproductive Biology
- Avian Physiology
Background:
- Light is crucial for avian reproduction, but mechanisms of light intensity's effect on ovarian development are unclear.
- Partridge broiler breeders' reproductive cycles are influenced by environmental light cues.
Purpose of the Study:
- To investigate the impact of varying light intensities on ovarian development and laying performance in partridge broiler breeders.
- To identify molecular mechanisms, including gene and metabolite changes, underlying light intensity's regulation of avian reproduction.
Main Methods:
- Partridge broiler breeders were divided into low-light (LL) and natural-light (NL) intensity groups.
- Serum hormone levels (P4, LH, E2, FSH) were measured at 22 and 32 weeks.
- Ovarian transcriptomics and metabolomics were employed to identify differentially expressed genes (DEGs) and metabolites (DMs).
Main Results:
- The LL group exhibited a delayed age at first egg and an extended laying period.
- Hormone levels differed between groups, with higher P4 and LH in LL at 22 wk, and lower P4, E2, LH, and FSH at 32 wk.
- Transcriptomic and metabolomic analyses revealed significant DEGs and DMs, highlighting pathways like steroid hormone biosynthesis and neuroactive ligand-receptor interaction.
Conclusions:
- Light intensity significantly influences partridge breeder reproductive timing and performance.
- Specific genes (e.g., CYP21A1, SSTR2, NPY) and metabolites (e.g., tryptamine, triglycerides, phenylalanine) are implicated in mediating light's effects on ovarian development.
- Findings provide insights into the molecular basis of light-intensity regulation in avian reproduction.
