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In Vitro Assay Development to Study Pulse Field Ablation Outcome Using Solanum Tuberosum.

Akshay Narkar1, Abouzar Kaboudian1, Yasaman Ardeshirpour1

  • 1Center for Devices and Radiological Health, US Food and Drug Administration, Silver Spring, MD 20993, USA.

International Journal of Molecular Sciences
|August 29, 2024
PubMed
Summary
This summary is machine-generated.

This study refines bench testing for Pulsed Field Ablation (PFA) using potato tubers to assess ablation outcomes. It establishes an electroporation threshold, enabling rapid screening of PFA device parameters.

Keywords:
electroporationlesion depthpotatopulsed field ablationtuber model

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Area of Science:

  • Biophysics
  • Biotechnology
  • Medical Device Testing

Background:

  • Electroporation uses electric fields to alter cell permeability, with applications in cancer and cardiac arrhythmia treatments as irreversible electroporation (IRE) or Pulsed Field Ablation (PFA).
  • Comprehensive characterization of PFA pulse parameters is crucial for ablation device testing to determine lesion boundaries and efficacy, while minimizing animal burden.
  • The potato tuber is a common model for initial electroporation testing.

Purpose of the Study:

  • To characterize and refine bench testing methods for evaluating Pulsed Field Ablation (PFA) outcomes in a potato tuber model.
  • To investigate the effects of various pulse parameters (voltage, duration, frequency) on 2D ablation area, 3D depth, and volume.
  • To measure temperature changes during PFA and supplement experimental data with in silico modeling of electric field distribution.

Main Methods:

  • In vitro assays were conducted on potato tubers, modulating voltage, duration, and frequency of electric field pulses.
  • Measurements included 2D ablation area, 3D depth, volume, and temperature changes before, during, and after ablation.
  • In silico modeling was used to analyze electric field distribution, complementing experimental findings.

Main Results:

  • The irreversible electroporation threshold in *Solanum Tuberosum* was estimated to be 240 V/cm.
  • The study characterized the impact of different pulse parameters on ablation outcomes in the potato model.
  • Minimal thermal effects were observed, consistent with PFA's characteristics.

Conclusions:

  • A refined bench testing platform using potato tubers allows for rapid, high-throughput screening of PFA pulse parameters.
  • This approach facilitates early-stage PFA device development, reducing the need for extensive animal trials.
  • The established electroporation threshold provides a critical benchmark for PFA device efficacy assessment.