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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Comprehensive Analysis of Methylome and Transcriptome to Identify Potential Genes Regulating Porcine Testis

Yue Feng1, Yu Zhang1, Junjing Wu1

  • 1Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.

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This study reveals key DNA methylation patterns in developing pig testes, identifying genes crucial for spermatogenesis. Findings highlight how DNA methylation regulates gene expression during testicular development.

Keywords:
DNA methylationRNA-SeqWGBSporcinetesticular development

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Area of Science:

  • Epigenetics
  • Reproductive Biology
  • Genomics

Background:

  • DNA methylation is vital for gene expression regulation in testicular development.
  • Limited research exists on DNA methylation's role in porcine testicular development.

Purpose of the Study:

  • To investigate differentially expressed genes (DEGs) and DNA methylation levels in porcine testicular tissues at different developmental stages.
  • To identify candidate genes involved in the epigenetic regulation of porcine testicular development.

Main Methods:

  • RNA sequencing (RNA-Seq) and whole genome bisulfite sequencing (WGBS) were employed.
  • Analysis focused on cytosine-guanine (CG) methylation context.
  • Integrated analysis of RNA-Seq and WGBS data identified differentially methylated genes (DMGs) correlated with gene expression.

Main Results:

  • 106,282 differentially methylated regions (DMRs) and 12,385 differentially methylated genes (DMGs) were identified.
  • 1083 DMGs showed a negative correlation with DEGs, enriched in spermatogenesis and germ cell development pathways.
  • Specific genes like ADAM30, ADAM3A, YBX2 (repressed by hyper-methylation) and CACNA1I, CTNNB1, JAM2 (activated by hypo-methylation) were highlighted.

Conclusions:

  • DNA methylation significantly influences gene expression during porcine testicular development.
  • Identified DMGs provide insights into epigenetic regulatory mechanisms governing spermatogenesis.
  • This study establishes a foundation for understanding epigenetic control in livestock reproduction.