Rosmarinic Acid Potentiates Cytotoxicity of Cisplatin against Colorectal Cancer Cells by Enhancing Apoptotic and Ferroptosis
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View abstract on PubMed
Summary
This summary is machine-generated.Rosmarinic acid enhances cisplatin's anticancer effect on colon cancer cells by promoting apoptosis and ferroptosis. This combination therapy offers a promising strategy for colorectal cancer treatment.
Area Of Science
- Oncology
- Pharmacology
- Molecular Biology
Background
- Rosmarinic acid (RA) exhibits anticancer properties against various cancers.
- The synergistic effect of RA with cisplatin on colorectal cancer remains underexplored.
Purpose Of The Study
- To investigate if RA potentiates cisplatin's cytotoxicity in colon cancer cells.
- To elucidate the underlying molecular mechanisms of this combined effect.
Main Methods
- Cell viability assessed via sulforhodamine B (SRB) assay.
- Cell cycle progression and apoptosis analyzed by flow cytometry and Annexin V staining.
- Western blotting used to examine key signaling pathways.
Main Results
- RA significantly boosted cisplatin's inhibition of cell viability and induced apoptosis in DLD-1 and LoVo cells.
- Combined RA and cisplatin upregulated Bax and caspase activation, while downregulating Bcl-2, GPX4, and SLC7A11.
- Inhibitors of caspases and ferroptosis partially reversed the combined treatment's effect on cell viability.
Conclusions
- Rosmarinic acid enhances cisplatin's anticancer efficacy in colon cancer.
- The potentiation involves the induction of apoptosis and ferroptosis through specific molecular pathways.
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