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Related Experiment Videos

One gene encodes two distinct Ia-associated invariant chains.

K Yamamoto, N Koch, M Steinmetz

    Journal of Immunology (Baltimore, Md. : 1950)
    |May 1, 1985
    PubMed
    Summary

    A single gene encodes two related immune response proteins, the invariant (Ii) chain and a 41K protein. These proteins share serological and compositional similarities, originating from distinct precursors.

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Murine immune response region-encoded Ia antigens are complex.
    • These antigens associate with the invariant (Ii) chain and other polypeptides, including a notable 41K protein.

    Purpose of the Study:

    • To investigate the relationship between the invariant (Ii) chain and the 41K protein.
    • To determine if a single gene could encode both polypeptides.

    Main Methods:

    • Serological cross-reactivity testing.
    • Partial enzymatic digestion to compare peptide composition.
    • Tunicamycin treatment and cell-free translation to analyze precursor forms.
    • Hybrid selection of mRNA and Northern blotting using Ii cDNA.
    • Transfection of rat fibroblasts with a mouse genomic Ii clone.

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    Main Results:

    • The invariant (Ii) chain and 41K protein demonstrated serological cross-reactivity.
    • Similar peptide compositions were observed after partial enzymatic digestion.
    • Both polypeptides were derived from distinct unglycosylated precursors.
    • Two distinct mRNAs coding for the Ii chain and 41K protein were identified.
    • Transfected fibroblasts synthesized both murine Ii chain and 41K protein.

    Conclusions:

    • A single invariant chain (Ii) gene encodes two distinct but closely related proteins.
    • The invariant chain and 41K protein are products of the same genetic locus.
    • This finding provides new insights into the complexity of Ia antigen-associated proteins.