Development of Proniosome Gel Formulation for CHIKV Infection

Area of Science:

• Pharmacology
• Drug Delivery Systems
• Rheumatology

Background:

• Musculoskeletal diseases and arthritis are increasing due to aging and obesity.
• Chikungunya virus (CHIKV) exacerbates arthritis, necessitating novel treatments.
• Berberine shows therapeutic potential but suffers from poor bioavailability and stability.

Purpose of the Study:

• To develop and evaluate a berberine-loaded proniosome gel for topical application.
• To enhance berberine's bioavailability and efficacy for treating CHIKV-induced arthritis.
• To investigate the physical properties, stability, skin permeability, and biological activity of the proniosome gel.

Main Methods:

• Formulation of proniosome gel with varying berberine loads and excipients.
• Assessment of physical properties, stability, and skin permeation.
• Evaluation of in vivo pharmacokinetic profile in mice.
• In vitro analysis of antioxidant stress, cell viability, and anti-inflammatory effects.

Main Results:

• Proniosome gel formulation optimized for stability and skin permeability.
• Demonstrated sustained release and enhanced bioavailability of berberine.
• Showcased significant antioxidant and anti-inflammatory activities in vitro.
• Potential for topical treatment of CHIKV infection and associated arthritis.

Conclusions:

• Topical berberine proniosome gel offers a promising strategy for arthritis treatment.
• Enhanced bioavailability and stability improve therapeutic potential.
• This approach could provide a novel alternative for managing CHIKV-related joint inflammation.