Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Adhesion in Plants01:14

Cell Adhesion in Plants

2.6K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.6K
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

3.5K
 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
3.5K
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K
Plant Cell Wall02:43

Plant Cell Wall

56.4K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.4K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.8K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

72.1K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
72.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment outcomes of fruquintinib combined with PD-1 inhibitors in advanced colorectal cancer: a reconstructed patient-level meta-analysis of retrospective real-world studies.

BMC cancer·2026
Same author

Beyond knowledge deficits: social visibility, family dynamics, and delayed initiation among people living with HIV in Southwestern China-a descriptive phenomenological study.

BMC public health·2026
Same author

Sugarcane smut fungus hijacks the host meristem: phytohormone-mediated sorus morphogenesis and metabolic reprogramming.

Frontiers in microbiology·2026
Same author

Intratumoral bicarbonate functions as an adjuvant to potentiate PD-1 blockade in hepatocellular carcinoma.

Oncogene·2026
Same author

Novel <i>FLCN</i> mutations in Birt-Hogg-Dubé patients and potential intervention of <i>FLCN</i> mRNA.

Therapeutic advances in respiratory disease·2026
Same author

Traditional Chinese Medicine-Derived Active Ingredient and Formulation Therapy for Glioma: Multi-Target Mechanisms, Drug Delivery Systems, and Advances in Clinical Translational Research.

Pharmaceuticals (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 14, 2025

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

13.7K

Plant Cell Wall Polysaccharide O-Acetyltransferases.

Ruiqin Zhong1, Dayong Zhou2, Lirong Chen2

  • 1Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.

Plants (Basel, Switzerland)
|August 29, 2024
PubMed
Summary

Plant cell wall O-acetylation, crucial for assembly and defense, is performed by TBL/DUF231 O-acetyltransferases. Understanding these enzymes offers insights into plant biology and potential biofuel applications.

Keywords:
TBLacetyltransferasecell wallmannanpectinxylanxyloglucan

More Related Videos

Glycan Profiling of Plant Cell Wall Polymers using Microarrays
12:30

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

14.6K
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

9.6K

Related Experiment Videos

Last Updated: Jun 14, 2025

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

13.7K
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
12:30

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

14.6K
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
11:06

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

9.6K

Area of Science:

  • Plant Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Plant cell walls are complex structures primarily composed of polysaccharide polymers.
  • Key polysaccharides like xyloglucan, xylan, mannan, and pectins undergo O-acetylation.
  • Polysaccharide O-acetylation is vital for cell wall assembly and plant disease resistance.

Purpose of the Study:

  • To provide a comprehensive overview of O-acetylated cell wall polysaccharides.
  • To detail the biochemical properties, structural features, and evolution of O-acetyltransferases.
  • To explore potential biotechnological applications of manipulating cell wall acetylation.

Main Methods:

  • Genetic and biochemical analyses were used to identify key protein families involved in O-acetylation.
  • Literature review and comparative analysis of O-acetyltransferase families (TBL/DUF231, RWA, AXY9).
  • Examination of the occurrence and function of O-acetylated polysaccharides.

Main Results:

  • The trichome birefringence-like (TBL)/domain of unknown function 231 (DUF231) protein family acts as O-acetyltransferases for xyloglucan, xylan, mannan, and pectins.
  • While the roles of Reduced Wall Acetylation (RWA) and Altered Xyloglucan 9 (AXY9) proteins are still under investigation, TBL/DUF231 proteins are confirmed O-acetyltransferases.
  • O-acetylation is a widespread modification in plant cell walls with significant functional implications.

Conclusions:

  • TBL/DUF231 proteins are the primary O-acetyltransferases responsible for modifying diverse plant cell wall polysaccharides.
  • Further research into O-acetylation mechanisms will enhance understanding of cell wall biology.
  • Manipulating cell wall acetylation holds promise for engineering disease resistance and improving biofuel production efficiency.