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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 18, 2026

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Published on: June 13, 2014

Gold nanorod-based smart platform for efficient cellular uptake and combination therapy.

Kibeom Kim¹, Mamta Ramgopal Chejara^1,2,3, Been Yoon^2,3

¹Department of Chemistry and Life Science, Sahmyook University Seoul 01795 South Korea mpark@syu.ac.kr.

|August 29, 2024

Summary

This study introduces a novel gold nanorod (GNR) platform coated with amphiphilic polymers for combined chemotherapy and photothermal therapy. This advanced system enhances drug delivery and cancer treatment efficacy through targeted approaches.

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Last Updated: Jun 18, 2026

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

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Published on: June 13, 2014

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Published on: January 15, 2018

Area of Science:

Biomedical Engineering
Nanotechnology
Materials Science

Background:

Gold nanorods (GNRs) show promise as drug carriers due to biocompatibility and targeting capabilities.
Limitations include drug structural changes, limited drug loading capacity, and small internal space compared to porous materials.

Purpose of the Study:

To develop an amphiphilic polymer-coated GNR platform for combined chemo- and photothermal therapy.
To overcome limitations of traditional GNR drug delivery systems.
To enhance cancer treatment efficacy through synergistic therapeutic approaches.

Main Methods:

Fabrication of GNRs coated with amphiphilic polymers containing hydrophobic chains, polyethylene glycol, and folic acid.
Utilizing near-infrared (NIR) light to induce photothermal heating for controlled drug release and enhanced cellular uptake.
Quantifying drug release kinetics using Nile red and assessing targeting and delivery efficiency with paclitaxel in cellular experiments.

Main Results:

The GNR platform demonstrated controlled drug release upon NIR irradiation.
Folic acid functionalization facilitated active targeting of cancer cells.
The combination therapy showed enhanced efficacy in cellular experiments, confirming targeting and delivery efficiency.

Conclusions:

The developed amphiphilic polymer-coated GNR platform is effective for chemo- and photothermal combination therapy.
This platform offers advantages in drug encapsulation, controlled release, and targeted delivery for improved cancer treatment.
The system integrates passive and active targeting strategies to maximize therapeutic outcomes.