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Published on: January 28, 2020
A nomogram prediction of coronary artery dilation in Kawasaki diseases based on mtDNA copy number
Mou Peng1, Peng Yue1, Yue Zhang1
1Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education (MOE), Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Insights
Mitochondrial DNA copy number (mtDNA-CN) is elevated in children with Kawasaki disease (KD) and is a significant predictor of coronary artery dilation. Higher mtDNA-CN levels indicate increased risk for KD complications.
Area of Science:
- Biochemistry
- Immunology
- Pediatrics
Background:
- Mitochondrial DNA copy number (mtDNA-CN) in peripheral blood cells is implicated in immune and cardiovascular diseases.
- Kawasaki disease (KD) is a critical pediatric illness associated with cardiovascular complications, particularly coronary artery lesions.
Purpose of the Study:
- To evaluate mtDNA-CN levels in children diagnosed with KD.
- To develop a nomogram for predicting coronary artery lesions in pediatric KD patients.
Main Methods:
- Assessed clinical features and laboratory parameters in 144 children with KD and compared them to a healthy group.
- Performed univariable and multivariable analyses to identify risk factors for coronary artery dilation.
- Constructed a predictive nomogram based on identified independent risk factors.
Main Results:
- Children with KD exhibited significantly higher mtDNA-CN levels compared to healthy controls (P<0.001).
- mtDNA-CN, Red Blood Cell count (RBC), and Platelet count (PA) were identified as independent risk factors for coronary artery dilation in KD.
- A nomogram demonstrated satisfactory predictive and calibration values for coronary artery lesions.
Conclusions:
- Elevated mtDNA-CN serves as a potential biomarker for predicting Kawasaki disease development.
- Higher mtDNA-CN levels are significantly associated with the presence of coronary artery dilation in children with KD.
Objective:
The level of mitochondrial DNA copy number (mtDNA-CN) in peripheral blood cells had been identified to be involved in several immune and cardiovascular diseases. Thus, the aim of this study is to evaluate the levels of mtDNA-CN in Kawasaki disease (KD) and to construct a nomogram prediction for coronary artery lesions in children with KD.
Methods:
One hundred and forty-four children with KD diagnosed from March 2020 to March 2022 were involved in the study. The clinical features and laboratory test parameters of these children were assessed between the KD and normal groups. Univariable and multivariable analyses were performed sequentially to identify the essential risk factors. Subsequently, a nomogram prediction was constructed.
Results:
A total of 274 children were included in the analysis. Of these, 144 (52.6%) represented the KD group. Peripheral blood DNA mtDNA qPCR showed that the -log value of mtDNA-CN in the KD group (6.67 ± 0.34) was significantly higher than that in the healthy group (6.40 ± 0.18) (P<0.001). The area under the ROC curve for mtDNA-CN in distinguishing KD was 0.757. MtDNA-CN (OR = 13.203, P = 0.009, 95% CI 1.888-92.305), RBC (OR = 5.135, P = 0.014, 95% CI 1.394-18.919), and PA (OR = 0.959, P = 0.014, 95% CI 0.927-0.991) were identified as independent risk factors for coronary artery dilation in children with KD. Finally, the nomogram predictive was established based on the results of multivariable analysis, demonstrating the satisfied prediction and calibration values.
Conclusion:
The results of this study revealed that mtDNA-CN could be used as a biomarker in predicting the development of KD. Furthermore, the higher the mtDNA-CN was significantly associated with coronary artery dilation in KD.
