Related Experiment Video
Updated: Jun 14, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10
Jinglan Hao1, Guiqin Han1, Xin Liang1
1College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Abstract:
Erythropoiesis is a multistep process of erythroid cell production that is controlled by multiple regulatory factors. Ribosome rescue factor PELO plays a crucial role in cell meiotic division and mice embryonic development. However, the function of PELO in erythroid differentiation remains unclear. Here, we showed that knockdown of PELO increased hemin-induced erythroid differentiation of K562 and HEL cells, exhibiting a higher number of benzidine-positive cells and increased mRNA levels of erythroid genes. PELO knockdown inhibited the proliferation and cell cycle progression and promoted apoptosis of K562 cells. Mechanistically, PELO could regulate the expression of KLF10 through interaction with MYC. Moreover, KLF10 knockdown also enhanced erythroid differentiation of K562 and HEL cells induced by hemin. Collectively, our results demonstrated that PELO regulates erythroid differentiation and increases KLF10 expression levels by interacting with MYC.
More Related Videos
12:44Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Published on: November 11, 2014
15:32Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
Published on: August 5, 2011
Related Concept Videos
Master Transcription Regulators
Somatic to iPS Cell Reprogramming
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
Methods of Nuclear Reprogramming
General Transcription Factors
RNA Polymerase II Accessory Proteins