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Updated: Jun 14, 2025

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Circ_0076490 silencing inhibits MAPK1 expression to decrease the proliferation and increase apoptosis of Jurkat cells

Qin Ye1, Chengyao Gu2, Wang Yan2

  • 1Department of Pulmonary, Ningbo No.2 Hospital, Ningbo, China.

Neurological Research
|August 29, 2024
PubMed
Abstract

Insights

Circular RNA circ_0076490 is upregulated in myasthenia gravis (MG) and drives disease progression by inhibiting miR-144-3p, which in turn activates MAPK1. Targeting circ_0076490 offers a potential therapeutic strategy for MG.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Myasthenia gravis (MG) is an autoimmune disorder affecting the neuromuscular junction.
  • The pathogenesis of MG involves circular RNAs (circRNAs), but the specific role of circ_0076490 remains unclear.

Purpose of the Study:

  • To investigate the role of circ_0076490 in myasthenia gravis.
  • To elucidate the underlying molecular mechanism involving miR-144-3p and MAPK1.

Main Methods:

  • Quantitative real-time PCR to measure RNA levels.
  • Cell proliferation, cell cycle, and apoptosis assays (CCK-8, EdU, flow cytometry).
  • Bioinformatic prediction and experimental validation (dual-luciferase, RNA pull-down) of RNA interactions.

Main Results:

  • Circ_0076490 and MAPK1 expression were elevated, while miR-144-3p was decreased in MG patients.
  • Circ_0076490 knockdown inhibited Jurkat cell proliferation and promoted apoptosis.
  • Circ_0076490 targets miR-144-3p, which targets MAPK1, forming a regulatory axis.

Conclusions:

  • Circ_0076490 knockdown inhibits proliferation and induces apoptosis in Jurkat cells via the miR-144-3p/MAPK1 pathway.
  • This circ_0076490/miR-144-3p/MAPK1 axis represents a potential therapeutic target for myasthenia gravis.
Keywords:
Jurkat cellsMAPK1MGcirc_0076490miR-144-3p

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