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Updated: Jun 14, 2025

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
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Downregulation of SSR2 Enhances Hepatocellular Carcinoma Cisplatin Sensitivity via the Hippo Pathway

Rong Ye1, Ling Yin2, Yilong Ge3

  • 1Department of General Surgery III, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China.

Frontiers in Bioscience (Landmark Edition)
|August 29, 2024
PubMed
Abstract

Insights

Signal sequence receptor subunit 2 (SSR2) upregulation promotes hepatocellular carcinoma (HCC) progression and chemoresistance. Targeting the SSR2/Hippo axis may enhance chemotherapy effectiveness in HCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Hepatocellular carcinoma (HCC) chemoresistance limits patient survival.
  • Novel therapeutic targets are needed to overcome HCC chemotherapy resistance.

Purpose of the Study:

  • To investigate the role of Signal sequence receptor subunit 2 (SSR2) in HCC.
  • To explore SSR2 as a potential therapeutic target for enhancing HCC chemotherapy.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qPCR) and Western blotting for SSR2 expression.
  • Assays for cell proliferation, apoptosis, and anchorage-independent growth.
  • In vivo animal models and molecular mechanism studies (luciferase reporter assays).

Main Results:

  • SSR2 is upregulated in HCC and linked to poor patient survival.
  • Downregulation of SSR2 increases HCC sensitivity to Cisplatin (DDP).
  • SSR2 inhibits YAP phosphorylation, promoting Hippo pathway gene transcription.

Conclusions:

  • SSR2 plays a critical role in HCC progression through the Hippo pathway.
  • Targeting the SSR2/Hippo axis is a potential strategy to overcome DDP resistance in HCC.
Keywords:
DDPHCCHippo pathwaySSR2chemotherapy resistance

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