Related Experiment Video
Updated: Jun 14, 2025
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
Complete genome sequence of Vibrio cholerae LK-18 isolated from tail-rotted Procambarus clarkii
Mingshu Yang1, Jinxian Lv2, Kai Li2
1College of Food Science and Engineering, Hainan Tropical Ocean University; Marine Food Engineering Technology Research Center of Hainan Province; Collaborative Innovation Center of Marine Food Deep Processing, Sanya, China.
Abstract:
Isolated from tail-rotted Procambarus clarkii, the pathogenic bacterium Vibrio cholerae LK-18 features two circular chromosomes: chromosome I (2,895,335 bp) and chromosome II (1,175,190 bp). The genome includes 3,522 open reading frames, 100 tRNA genes, and 31 rRNA genes, and it harbors the Vibrio cholera cytolysin and chitinase genes.
Insights
The pathogenic bacterium Vibrio cholerae LK-18, isolated from diseased crayfish, possesses a two-chromosome genome. This genome contains genes for virulence factors like Vibrio cholerae cytolysin and chitinase.
Area of Science:
- Microbiology
- Genomics
- Aquatic Animal Health
Background:
- Vibrio cholerae is a significant pathogen affecting aquatic organisms.
- Tail rot in Procambarus clarkii is a disease of economic and ecological concern.
- Understanding the genomic features of V. cholerae strains is crucial for disease management.
Purpose of the Study:
- To characterize the genome of the Vibrio cholerae LK-18 strain.
- To identify potential virulence factors present in the LK-18 strain.
- To provide genomic insights into V. cholerae pathogenicity in Procambarus clarkii.
Main Methods:
- Isolation and identification of the bacterial strain LK-18 from infected Procambarus clarkii.
- Whole-genome sequencing of the Vibrio cholerae LK-18 isolate.
- Bioinformatic analysis to identify genes, including open reading frames (ORFs), tRNA, and rRNA genes.
Main Results:
- The Vibrio cholerae LK-18 genome consists of two circular chromosomes (Chromosome I: 2,895,335 bp; Chromosome II: 1,175,190 bp).
- The genome contains 3,522 open reading frames, 100 tRNA genes, and 31 rRNA genes.
- Key genes identified include those encoding Vibrio cholerae cytolysin and chitinase.
Conclusions:
- The genomic analysis reveals the complete structure of the Vibrio cholerae LK-18 genome.
- The presence of cytolysin and chitinase genes suggests potential virulence mechanisms.
- This study contributes to understanding the genetic basis of V. cholerae infections in crayfish.
