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DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

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Simple Analysis of Gel Images With IOCBIO Gel Software.

Lucia Jaska¹, Rikke Birkedal¹, Martin Laasmaa¹

¹Laboratory of Systems Biology, Department of Cybernetics, Tallinn University of Technology, Tallinn, Estonia.

|August 30, 2024

Summary

Reproducible gel image analysis is crucial for scientific integrity. IOCBIO Gel software offers a free, open-source solution for electrophoresis image analysis, ensuring accurate and repeatable results.

Keywords:

Data analysis Electrophoresis gel FAIR Isoelectric focusing Python Reproducibility Southern blotting Western blotting

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Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

Gel image analysis is critical for various electrophoresis techniques.
Current software like ImageJ Gels plugin lacks automated step recording, hindering reproducibility.
Difficulty in reproducing gel image analysis results impacts scientific validity.

Purpose of the Study:

To provide a detailed protocol for reproducible gel image analysis using IOCBIO Gel software.
To demonstrate the applicability of IOCBIO Gel for various electrophoresis-based imaging.
To highlight the advantages of IOCBIO Gel over existing solutions.

Main Methods:

Detailed step-by-step protocol for gel image analysis using IOCBIO Gel.
Demonstration with western blot as a primary example.
Applicability extended to Southern blotting, northern blotting, and isoelectric focusing.

Main Results:

IOCBIO Gel software enables reproducible gel image analysis.
The software links original images with all performed operations, allowing corrections.
It supports multiple sample analyses and data storage for easy access.

Conclusions:

IOCBIO Gel provides a free, open-source, and lightweight solution for electrophoresis gel image analysis.
The software enhances reproducibility and accessibility of gel image analysis.
IOCBIO Gel is a versatile tool applicable to a wide range of electrophoresis imaging needs.