Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Medium-Chain Versus Medium- and Long-Chain Triglycerides, Combined with Carotenoids, in a High-Fat Diet on Obese Mice.

Foods (Basel, Switzerland)·2026
Same author

Preparation of solid-state emissive carbon quantum dots and their integration into electroluminescent light-emitting diodes.

Nature protocols·2026
Same author

Unlocking Interfacial Binder Chemistry for Efficient Li<sup>+</sup> Desolvation in 3C-Rate Lithium Metal Pouch Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

From disordered dots to coherent pixels: superlattice ordering enables high-performance perovskite LEDs.

Science bulletin·2026
Same author

Day-Long Persistent Luminescence in Intrinsically Integrated Donor-Acceptor Carbon Dots Enabled by Defect-Mediated Charge Trapping.

Angewandte Chemie (International ed. in English)·2026
Same author

Highly Dissymmetric and Multicolor Circularly Polarized Organic Hyperafterglow.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Jun 14, 2025

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.1K

Engineered Protein-G variants for plug-and-play applications.

Tomasz Slezak, Kelly M O'Leary, Jinyang Li

    Biorxiv : the Preprint Server for Biology
    |August 30, 2024
    PubMed
    Summary

    Researchers created modular antibody building blocks for rapid assembly of complex biological tools. This plug-and-play system enables fast creation of custom antibody assemblies for diverse cell biology applications.

    More Related Videos

    Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
    07:35

    Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

    Published on: June 14, 2021

    2.7K
    High-throughput Protein Expression Generator Using a Microfluidic Platform
    09:26

    High-throughput Protein Expression Generator Using a Microfluidic Platform

    Published on: August 23, 2012

    11.7K

    Related Experiment Videos

    Last Updated: Jun 14, 2025

    Protein Engineering by Yeast Surface Display
    05:49

    Protein Engineering by Yeast Surface Display

    Published on: November 29, 2024

    1.1K
    Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
    07:35

    Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

    Published on: June 14, 2021

    2.7K
    High-throughput Protein Expression Generator Using a Microfluidic Platform
    09:26

    High-throughput Protein Expression Generator Using a Microfluidic Platform

    Published on: August 23, 2012

    11.7K

    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Immunology

    Background:

    • Antibody engineering is crucial for developing advanced diagnostics and therapeutics.
    • Current methods for creating multifunctional antibody assemblies can be time-consuming and complex.
    • There is a need for versatile, modular systems to streamline antibody-based reagent production.

    Purpose of the Study:

    • To develop a modular, plug-and-play platform for assembling multifunctional antibody-based reagents.
    • To engineer high-specificity antibody fragments (Fabs) and protein G (PG) variants for orthogonal pairing.
    • To demonstrate the platform's utility in creating diverse antibody formats, including IgG-like assemblies, bispecific antibodies, and Bispecific T-cell Engagers (BiTEs).

    Main Methods:

    • Utilized phage display to engineer specific Fab-PG pairings.
    • Developed prefabricated antibody modules and PG variants fused to SNAP-tags for multiplexed detection.
    • Constructed IgG-like assemblies and bispecific antibodies using PG-Fc dimer core units and the "knobs into holes" strategy.
    • Incorporated commercial antibodies as building blocks.
    • Performed crystal structure analysis to understand specificity-determining interactions.

    Main Results:

    • Successfully created a portfolio of antibody modules with high pair-wise specificity.
    • Demonstrated rapid assembly of customized IgG-like antibodies and bispecific analogs in minutes.
    • Showcased the application of bispecific modalities in fabricating potent Bispecific T-cell Engagers (BiTEs) with demonstrated cancer cell-killing efficacy.
    • Confirmed the system's adaptability to include commercial antibodies, expanding target accessibility.
    • Structural analysis revealed key interactions responsible for scaffold specificity.

    Conclusions:

    • The developed plug-and-play antibody module platform offers a user-friendly and versatile approach for creating complex antibody assemblies.
    • This system significantly accelerates the production of customized antibody reagents, reducing timelines from weeks to minutes.
    • The platform holds substantial promise for advancing cell biology research, diagnostics, and therapeutic development through enhanced antibody functionality.