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Rational Design of Pyrido[3,2-b]indolizine as a Tunable Fluorescent Scaffold for Fluorogenic Bioimaging.
Sihyeong Yi1, Dahham Kim1, Wansang Cho1
1CRI Center for Chemical Proteomics, Department of Chemistry, Seoul National University, Seoul 08826, Korea.
JACS Au
|August 30, 2024
Summary
Researchers developed novel pyrido[3,2-b]indolizine fluorophores for biomedical imaging. One fluorophore enables washing-free visualization of cellular lipid droplets in living cells.
Area of Science:
- Organic chemistry
- Biomedical imaging
- Fluorescence spectroscopy
Background:
- Novel fluorescent scaffolds are crucial for biomedical research.
- Existing fluorophores often have limitations in size, emission color, or application scope.
Purpose of the Study:
- To design and synthesize a versatile organic fluorophore based on the pyrido[3,2-b]indolizine scaffold.
- To develop novel bioprobes for cellular imaging and peptide-protein interaction monitoring.
Main Methods:
- Computational modeling was used to design fluorophores with tunable emission.
- Synthesis of pyrido[3,2-b]indolizine derivatives.
- Development of fluorescent probes for cellular lipid droplet visualization and peptide-protein interaction studies.
Main Results:
- Constructed small-sized (<300 g/mol) fluorophores with visible light absorption (>400 nm) and tunable emission (blue to red).
- Discovered a highly fluorogenic turn-on fluorophore (1) for washing-free visualization of cellular lipid droplets.
- Developed tryptophan-analogous fluorogenic unnatural amino acids for peptide probes.
Conclusions:
- The pyrido[3,2-b]indolizine scaffold is a promising platform for developing advanced organic fluorophores.
- The developed probes offer new tools for live-cell imaging and studying biomolecular interactions.
