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Low-Frequency Coherent Raman Imaging Robust to Optical Scattering
David R Smith1, Jesse W Wilson2,3, Siddarth Shivkumar4,5
1Biomedical Imaging, Morgridge Institute for Research, Madison, Wisconsin 53715, United States.
Chemical & Biomedical Imaging
|August 30, 2024
Summary
We developed a robust impulsive stimulated Raman scattering (ISRS) imaging method that overcomes scattering issues. This technique successfully captures high-quality Raman spectra from complex, scattering samples.
Area of Science:
- Spectroscopy
- Microscopy
- Optics
Background:
- Impulsive stimulated Raman scattering (ISRS) captures vibrational spectra using pump-probe spectroscopy.
- Extracting ISRS spectra requires separating probe pulses from pump pulses, which is challenging in scattering media.
- Optical scattering in samples contaminates the probe pulse, hindering Raman spectral acquisition.
Purpose of the Study:
- To develop a robust ISRS imaging method for complex scattering samples.
- To overcome pump pulse contamination in ISRS measurements.
- To enable high signal-to-noise Raman spectral acquisition in scattering environments.
Main Methods:
- Demonstration of low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging.
- Implementation of a novel method to isolate probe pulses from scattered pump pulses.
- Testing the technique on complex scattering samples with multiple scattering layers.
Main Results:
- Achieved high robustness to distortions caused by optical scattering.
- Successfully obtained high signal-to-noise ISRS spectra from scattering specimens.
- Demonstrated the capability of the method even when pump and probe pulses traverse numerous scattering layers.
Conclusions:
- The developed ISRS method provides a robust solution for vibrational spectroscopy in scattering samples.
- This technique significantly enhances the applicability of ISRS microscopy in biological and material science.
- High-quality Raman spectral data can be reliably obtained from previously inaccessible scattering media.
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