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Nine Cluster E mycobacteriophages isolated from soil.

Joseph M Gaballa1, Amanda Freise2, Krisanavane Reddi2

  • 1Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

Microbiology Resource Announcements
|August 30, 2024
PubMed
Summary

Nine mycobacteriophages were isolated from soil using Mycobacterium smegmatis. Genome analysis revealed these phages belong to Actinobacteriophage Cluster E, and their genomic features and similarities are discussed.

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bacteriophagesmycobacteriophage

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Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Mycobacteriophages are viruses that infect mycobacteria.
  • Actinobacteriophage Cluster E is a group of phages with specific genomic characteristics.

Purpose of the Study:

  • To isolate and characterize novel mycobacteriophages.
  • To analyze the genomes of newly isolated phages belonging to Actinobacteriophage Cluster E.
  • To identify similarities among these Cluster E phages.

Main Methods:

  • Isolation of mycobacteriophages from soil samples using Mycobacterium smegmatis as a host.
  • Genome sequencing of the isolated phages.
  • Bioinformatic analysis and annotation of phage genomes.

Main Results:

  • Nine mycobacteriophages (FireRed, MISSy, MPhalcon, Murica, Sassay, Terminus, Willez, YassJohnny, and Youngblood) were successfully isolated.
  • All isolated phages were identified as members of Actinobacteriophage Cluster E.
  • Genomic features and comparative analysis revealed intra-cluster similarities.

Conclusions:

  • The study expands the repertoire of characterized Actinobacteriophage Cluster E phages.
  • Genomic data provides insights into the evolutionary relationships within this phage cluster.
  • These findings contribute to the understanding of mycobacteriophage diversity and genomics.