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Analysis of tissue-substrate adhesion by hyperspectral surface plasmon resonance microscopy
Bo Yang1,2, Hongyi Tang1,2, Ziwei Liu3
1State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, 100190, China.
Analytical and Bioanalytical Chemistry
|August 30, 2024
Summary
This study introduces a hyperspectral surface plasmon resonance microscopy (HSPRM) system to quantify tissue-substrate adhesion in histology slides. The novel method accurately measures nanoscale adhesion distances, improving diagnostic accuracy.
Area of Science:
- Biomedical Engineering
- Materials Science
- Histopathology
Background:
- Histology slide preparation is crucial for accurate histopathology.
- Poor tissue-substrate adhesion can lead to diagnostic errors and failures.
- Quantitative analysis of tissue-slide adhesion remains a significant challenge.
Purpose of the Study:
- To develop and utilize a hyperspectral surface plasmon resonance microscopy (HSPRM) system for quantitative analysis of tissue-substrate adhesion.
- To investigate the nanoscale adhesion between mouse brain tissue sections and gold-coated glass slides.
- To demonstrate the potential of HSPRM for stain-free tissue imaging and improved histopathological diagnosis.
Main Methods:
- Fabrication of a laboratory-based hyperspectral surface plasmon resonance microscopy (HSPRM) system.
- Analysis of mouse brain tissue section adhesion on gold-coated glass slides.
- Utilizing single-pixel spectral sensing to obtain 2D distribution of resonance wavelengths (RWs).
- Applying a five-layer Fresnel reflection model to determine nanoscale water gap and adhesion distance (AD).
Main Results:
- Verified the existence of a nanoscale water gap between tissue sections and the substrate.
- Obtained a 2D image of tissue-substrate adhesion distance (AD).
- Measured AD of 20-35 nm in deionized water and 4-24 nm in saline solution.
- Demonstrated HSPRM's wide wavelength range (400-1000 nm) and high sensitivity, label-free detection capabilities.
Conclusions:
- The developed HSPRM system enables precise, quantitative measurement of tissue-substrate adhesion at the nanoscale.
- HSPRM offers significant potential for stain-free tissue imaging and accurate histopathological diagnosis.
- This technology can enhance diagnostic accuracy by addressing issues related to tissue section detachment.
