Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

43.6K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.6K
Antibiotic Selection00:57

Antibiotic Selection

52.4K
Overview
52.4K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.8K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

62.0K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.0K
Bacterial Signaling01:30

Bacterial Signaling

31.8K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
31.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wrinkle-like structures emerge from matrix complementarity and mechanical discontinuity in heterogenous biofilms.

NPJ biofilms and microbiomes·2026
Same author

Investigating the crystalline structure and structural heterogeneity of starch granules using polarization-based quantitative phase microscopy.

Food chemistry·2026
Same author

Emergent collective alignment gives competitive advantage to longer cells during range expansion.

Nature communications·2025
Same author

Tumor Spheroid Uptake of Fluorescent Nanodiamonds Is Limited by Mass Density: A 4D Light-Sheet Assay.

Chemical & biomedical imaging·2025
Same author

Genetic mixing and demixing on expanding spherical frontiers.

ISME communications·2024
Same author

Motility mediates satellite formation in confined biofilms.

The ISME journal·2023

Related Experiment Video

Updated: Jun 14, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

13.9K

Following plasmid propagation in complex bacterial communities.

Mireia Cordero1, Liselotte Jauffred1

  • 1The Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100 Copenhagen O, Denmark.

Cell Reports
|August 30, 2024
PubMed
Summary

Researchers discovered how bacterial colony organization affects gene transfer rates. This finding is crucial for understanding bacterial evolution and the spread of antibiotic resistance genes.

More Related Videos

Plasmid Stability Analysis with Open-Source Droplet Microfluidics
07:43

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

431
High-Resolution Comparison of Bacterial Conjugation Frequencies
05:18

High-Resolution Comparison of Bacterial Conjugation Frequencies

Published on: January 10, 2019

10.7K

Related Experiment Videos

Last Updated: Jun 14, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

13.9K
Plasmid Stability Analysis with Open-Source Droplet Microfluidics
07:43

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

431
High-Resolution Comparison of Bacterial Conjugation Frequencies
05:18

High-Resolution Comparison of Bacterial Conjugation Frequencies

Published on: January 10, 2019

10.7K

Area of Science:

  • Microbiology
  • Genetics
  • Evolutionary Biology

Background:

  • Bacterial colonies exhibit complex self-organization patterns.
  • Horizontal gene transfer (HGT) is a major driver of bacterial evolution and adaptation.
  • Understanding the regulation of HGT within colonies is essential.

Purpose of the Study:

  • To investigate the relationship between bacterial colony self-organization and the efficiency of horizontal gene transfer.
  • To identify regulatory mechanisms linking colony structure to gene transfer rates.

Main Methods:

  • Utilized surface-attached bacterial colony models.
  • Quantified rates of conjugation (a form of HGT).
  • Analyzed the selection dynamics of transconjugant populations.

Main Results:

  • Identified causative regulatory links between colony self-organization and conjugation rates.
  • Demonstrated that colony structure directly influences the frequency of gene transfer.
  • Observed subsequent selection of newly arising transconjugant bacteria.

Conclusions:

  • Bacterial colony architecture plays a critical role in regulating horizontal gene transfer.
  • Self-organization within colonies can modulate the spread of genetic material.
  • These findings provide insights into bacterial adaptation and evolution within structured communities.