Related Experiment Video
Updated: May 5, 2026
03:38
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
1.4K
Entropy-Driven Catalytic G-Quadruple Cycle Amplification Integrated with Ligases for Label-Free Detection of Single
Yunshan Zhang1, Fang Yang1,2, Tuo Huang1,2
1Research Center for Novel Computational Sensing and Intelligent Processing, Zhejiang Lab, Hangzhou 311121, China.
Analytical Chemistry
|August 30, 2024
Summary
This study introduces an entropy-driven catalytic (EDC) G-quadruplex (G4) amplification technology for sensitive, label-free detection of nucleic acids. The novel system overcomes weak fluorescence, enabling highly accurate single nucleotide polymorphism (SNP) identification.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- G-quadruplex/thioflavin (G4/THT) shows promise for label-free nucleic acid detection but suffers from weak fluorescence.
- Single-molecule G4/THT fluorescence efficiency limits its application scope.
Purpose of the Study:
- To develop a universal label-free signal amplification and output system for enhanced nucleic acid detection.
- To address the limitations of weak fluorescence in G4/THT systems.
- To create a sensitive and specific tool for single nucleotide polymorphism (SNP) detection.
Main Methods:
- Developed an entropy-driven catalytic (EDC) G4 (EDC-G4) cycle amplification technology.
- Integrated ligase chain reaction (LCR) for label-free SNP detection.
- Utilized G4/THT as a fluorescent luminophore for signal output.
Main Results:
- The LCR-EDC-G4 system achieved sensitive detection of mutant targets (MT) down to 22.39 fM.
- Demonstrated specific identification of 0.01% MT in mixed samples.
- Showcased potential application in real biological samples.
Conclusions:
- The EDC-G4 system effectively enhances G4/THT fluorescence for label-free detection.
- The LCR-EDC-G4 system offers a high-performance, practical tool for SNP detection.
- This work provides novel strategies for label-free fluorescent biosensing.
More Related Videos
Related Concept Videos
02:57Real Time RT-PCR
52.0K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
52.0K
03:00Next-generation Sequencing
88.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.0K