DNA-Origami-Based Precise Molecule Assembly and Their Biological Applications.

Area of Science:

• Biotechnology
• Nanotechnology
• Molecular Biology

Background:

• Natural biomolecule assembly inspires artificial precise molecule assembly.
• Precise control over molecular arrangement is crucial in chemistry, biology, and medicine.
• DNA origami offers a programmable nanoscale scaffold for molecular assembly.

Purpose of the Study:

• To review recent advancements in DNA origami-based precise molecule assembly.
• To highlight the assembly of diverse molecules and control over assembly parameters.
• To summarize emerging biological applications of DNA origami.

Main Methods:

• Introduction to DNA origami principles and capabilities.
• Review of recent literature (last three years) on DNA origami assembly.
• Categorization of assembled molecules and controlled parameters.

Main Results:

• DNA origami enables precise assembly of nucleic acids, proteins, organic molecules, and nanoparticles.
• Key parameters like distance, number, orientation, and pattern are precisely controlled.
• Significant progress in DNA origami applications across sensing, imaging, therapy, and biophysics.

Conclusions:

• DNA origami is a powerful tool for nanoscale molecular construction.
• Emerging biological applications demonstrate its versatility and potential.
• Future opportunities lie in addressing current challenges in the field.