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  11. Proteomics Analysis Of Renal Cell Line Caki-2 With Afmid Overexpression And Potential Biomarker Discovery In Urine

Proteomics Analysis of Renal Cell Line Caki-2 with AFMID Overexpression and Potential Biomarker Discovery in Urine

Jiameng Sun1, Jinchun Chang2,3, Zhengguang Guo1

  • 1Core Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.

Journal of Proteome Research
|August 30, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Aromatic caninurine formamase (AFMID) downregulation is linked to clear cell renal cell carcinoma (ccRCC). Overexpressing AFMID inhibits ccRCC cell growth and reveals potential diagnostic biomarkers.

Area of Science:

  • Biochemistry
  • Oncology
  • Metabolomics

Background:

  • Aromatic caninurine formamase (AFMID) is an enzyme in the tryptophan pathway.
  • AFMID is downregulated in clear cell renal cell carcinoma (ccRCC), but its role is unclear.

Purpose of the Study:

  • Investigate the role of AFMID in ccRCC tumorigenesis.
  • Identify potential urinary biomarkers for ccRCC diagnosis.

Main Methods:

  • AFMID overexpression in ccRCC cells.
  • Quantitative proteomics and urine proteomic analysis.
  • Biomarker validation in an independent cohort.

Main Results:

  • AFMID overexpression inhibited ccRCC cell proliferation.
  • Altered cellular signaling and metabolism pathways (lipid, inositol phosphate).
Keywords:
AFMIDRCCbiomarkerproteomics

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  • Identified a protein panel (APOC3, UMOD, CILP) with high diagnostic accuracy (AUC 0.883).

    • Conclusions:

    • AFMID plays an inhibitory role in ccRCC development.
    • AFMID-associated pathway changes are detectable in urine.
    • Discovered potential urinary biomarkers for ccRCC diagnosis and therapeutic targeting.
    urine