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Interferon gamma produced by mitogen-activated T lymphocytes does not directly mediate lymphoproliferation
European Journal of Immunology
|April 1, 1985
Summary
Human interferon gamma (IFN-gamma) does not appear essential for T lymphocyte proliferation. Neutralizing IFN-gamma with specific antibodies showed minimal impact on mitogen-induced lymphoproliferation, suggesting it
Area of Science:
- Immunology
- Cell Biology
Background:
- Interferon gamma (IFN-gamma) is a cytokine involved in immune responses.
- Its role in T lymphocyte proliferation during mitogenic stimulation is not fully understood.
Purpose of the Study:
- To investigate the necessity of endogenously produced IFN-gamma for mitogen-induced lymphoproliferation.
- To determine the effect of neutralizing IFN-gamma on T lymphocyte activation and responsiveness.
Main Methods:
- Human peripheral blood mononuclear cells and T lymphocyte-enriched cultures were stimulated with mitogens.
- Endogenous IFN-gamma was neutralized using specific polyclonal antisera (anti-L and anti-G).
- Lymphoproliferation was assessed by [3H]thymidine incorporation, and interleukin 2 responsiveness was measured.
Main Results:
- Neutralization of natural IFN-gamma (anti-L) caused small decreases in lymphoproliferation.
- Neutralization of recombinant IFN-gamma (anti-G) had minimal effect or slightly enhanced lymphoproliferation.
- Interleukin 2 responsiveness of T lymphocytes remained unaffected by anti-G.
Conclusions:
- Endogenously produced IFN-gamma is not a direct requirement for T lymphocyte proliferation.
- The specific antiserum used against IFN-gamma may influence observed effects on lymphoproliferation.