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Updated: Jun 14, 2025
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
Published on: March 4, 2015
Organotypic culture of post-mortem adult human brain explants exhibits synaptic plasticity
Yukiko Iwasaki1, Corentin Bernou2, Barbara Gorda2
1Univ Montpellier, Montpellier, France; UM-CNRS Laboratoire D'Informatique de Robotique et de Microelectronique de Montpellier (LIRMM), 161, Rue Ada, Montpellier, France; CNRS, Institut de Recherche en Infectiologie de Montpellier (IRIM), 1919 Route de Mende, Montpellier, France.
Background:
Synaptic plasticity is an essential process encoding fine-tuned brain functions, but models to study this process in adult human systems are lacking.
Objective:
We aim to test whether ex vivo organotypic culture of post-mortem adult brain explants (OPABs) retain synaptic plasticity.
Methods:
OPABs were seeded on 3D microelectrode arrays to measure local field potential (LFP). Paired stimulation of distant electrodes was performed over three days to investigate our capacity to modulate specific neuronal connections.
Results:
Long-term potentiation (LTP) or depression (LTD) did not occur within a single day. In contrast, after two and three days of training, OPABs showed a significant modulation of the paired electrodes' response compared to the non-paired electrodes from the same array. This response was alleviated upon treatment with dopamine.
Conclusion:
Our work highlights that adult human brain explants retain synaptic plasticity, offering novel approaches to neural circuitry in animal-free models.
