Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative efficacy of zanubrutinib plus obinutuzumab versus last prior treatment in relapsed/refractory follicular lymphoma: growth modulation index analysis from the ROSEWOOD study.

ESMO open·2026

Same author

Metabolic tumor volume predicts outcome in patients with advanced stage follicular lymphoma from the RELEVANCE trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2023

Same author

Report of consensus panel 2 from the 11th international workshop on Waldenström's macroglobulinemia on the management of relapsed or refractory WM patients.

Seminars in hematology·2023

Same author

Report of consensus panel 5 from the 11th international workshop on Waldenstrom's macroglobulinemia on COVID-19 prophylaxis and management.

Seminars in hematology·2023

Same author

Full automation of total metabolic tumor volume from FDG-PET/CT in DLBCL for baseline risk assessments.

Cancer imaging : the official publication of the International Cancer Imaging Society·2022

Same author

Progressive multifocal leukoencephalopathy post ibrutinib therapy in relapsed chronic lymphocytic leukaemia.

Leukemia & lymphoma·2022

Search research articles

Related Experiment Video

Updated: Jun 14, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Developing a Paired Whole Genome Sequencing Service for Children With Cancer.

L Sarkies¹, P Thomas¹, E A Edeko¹

¹Cambridge University Hospitals NHS Foundation Trust, UK.

Clinical Oncology (Royal College of Radiologists (Great Britain))

|August 30, 2024

Summary

Paired whole genome sequencing (PWGS) in pediatric oncology successfully identifies cancer predisposition syndromes. This approach ensures at-risk families receive genetic counseling and testing, improving cancer care equity.

Keywords:

Genomics Germline cancer predisposition Paired WGS Pediatrics

More Related Videos

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

Related Experiment Videos

Last Updated: Jun 14, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

Area of Science:

Genomic Medicine
Pediatric Oncology
Cancer Predisposition Syndromes

Background:

Paired (tumor and germline) whole genome sequencing (PWGS) is crucial for cancer diagnosis and management.
PWGS identifies oncogenic drivers, treatment targets, and hereditary cancer syndromes.
Identifying cancer predisposition syndromes has significant implications for patients and their families.

Purpose of the Study:

To describe the process of integrating PWGS into routine National Health Service (NHS) clinical care for pediatric cancer patients.
To share experiences and facilitate the establishment of similar services in other centers.
To lay the groundwork for future quantitative and qualitative research on PWGS in pediatric oncology.

Main Methods:

Descriptive article detailing the implementation of PWGS in clinical practice.
Audit of pediatric cancer patients with confirmed underlying cancer predisposition syndromes.

Main Results:

100% of families identified at risk for cancer predisposition syndromes were offered clinical genetics referral.
100% of at-risk first-degree relatives were offered predictive counseling and testing.
Improvement needed in documenting discussions on reproductive options (67% of families).

Conclusions:

Implementing audit recommendations will enhance the PWGS service.
Sharing experiences can encourage wider adoption of PWGS in pediatric oncology.
Further research is needed on long-term cancer risk reduction and psychosocial impact.