Rhizobacterial Community Structure Differs Between Landrace and Cultivar of Rice Under Drought Conditions

The rhizosphere plays a critical role in crop growth and fitness, particularly under moisture-stress conditions. Modern breeding has diminished the ability of crops to recruit beneficial microbiomes, making them more vulnerable to drought, unlike wild types and landraces that adapt better. This study aims to elucidate how rice landrace and cultivar influence the rhizosphere bacterial communities and biochemical attributes under normal and moisture stress conditions. Rhizospheres of rice landrace, Norungan, and high-yielding cultivar, Co51, were assessed using soil biochemical and 16S rRNA gene sequencing approaches. Drought negatively impacted soil carbon pools, enzymes, and respiration in the rhizospheres of both genotypes. However, Norungan's rhizosphere showed less harm than Co51's. During drought, reductions in soil organic carbon (3.94%), microbial biomass carbon (14.26%), labile carbon (1.94%), dehydrogenase (10.1%), urease (21.27%), phosphatase (9.61%), and respiration rate (15.02%) were more pronounced in Co51 than in Norungan. Alpha diversity of rhizosphere bacterial communities was significantly lower than bulk soil, with drought further reducing diversity in both genotypes. Drought decreased the abundance of Firmicutes and Bacteroidetes in Norungan's rhizosphere while it increased the abundance of Acidobacteria, Actinobacteria, Chloroflexi, and Proteobacteria. Conversely, in Co51's rhizosphere, drought enhanced the abundance of Firmicutes and Bacteroidetes while reducing the abundance of Acidobacteria and Proteobacteria. These results suggest that under moisture-stress conditions, the landrace Norungan recruits less-diversified, specific groups of microorganisms to augment the rhizosphere's functioning. This study underscores the need to develop strategies for cultivars and hybrids with enhanced rhizosphere resilience during drought conditions.