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Construction of a Three-Dimensional-Printed Immunosensing Platform Based on Smartphone Photothermal Signal
Min Wang1, Chaoyun Ma2, Mingzhe Jiang1
1School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832003, Xinjiang, China.
Analytical Chemistry
|August 31, 2024
Summary
This study presents a novel 3D-printed device for cost-effective biosensing, utilizing photothermal effects for sensitive detection of alpha-fetoprotein (AFP). The portable system offers a user-friendly approach for advancing precision and personalized medicine.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Photothermal effect combined with immunoassay offers a promising route for developing affordable and accessible biosensing platforms.
- Existing biosensing methods often face challenges in cost, user-friendliness, and precision, limiting their widespread application.
Purpose of the Study:
- To develop a novel, cost-effective, and user-friendly biosensing system by integrating three-dimensional (3D) printing technology with photothermal immunoassay.
- To design a portable device capable of precise sample handling and consistent optical measurements for enhanced detection accuracy.
- To detect alpha-fetoprotein (AFP) using methylene blue adsorbed by iron terephthalate (MB@MOF235) as a photothermal reagent.
Main Methods:
- Utilized 3D printing technology to create a novel structural support for a cell phone and laser probe, incorporating a three-way cavity for sample handling and precise positioning.
- Employed MB@MOF235 as the photothermal reagent for detecting AFP, leveraging the photothermal effect for signal generation.
- Validated the detection method through electrochemical testing, utilizing the electro-oxidizing activity of methylene blue (MB).
Main Results:
- Successfully developed a portable and user-friendly biosensing system for AFP detection.
- Achieved a wide detection range for AFP from 0.01 to 50 ng/mL.
- Established a lower detection limit (LOD) of 0.032 pg/mL for AFP, demonstrating high sensitivity.
- Confirmed the method's accuracy through electrochemical validation.
Conclusions:
- The developed 3D-printed photothermal immunoassay system provides a simple, portable, and visual method for sensitive AFP detection.
- This innovative approach serves as a reliable reference for advancing precision medicine towards personalized healthcare solutions.
- The integration of 3D printing and photothermal reagents offers a scalable and cost-effective strategy for next-generation biosensors.
