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Bioinformatics analysis of neutrophil-associated hub genes and ceRNA network construction in septic cardiomyopathy

Qingfei Cao1, Jing Li2, Meixue Chen2

  • 1Department of Urology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.

Aging
|August 31, 2024
PubMed

Insights

Septic cardiomyopathy (SCM) involves heart dysfunction during sepsis. This study identifies MRC1 as a key neutrophil-related gene involved in SCM, offering potential biomarkers for early diagnosis and treatment.

Area of Science:

  • Cardiology
  • Immunology
  • Molecular Biology

Background:

  • Septic cardiomyopathy (SCM) is a serious sepsis complication causing cardiac depression.
  • Neutrophils play a role in sepsis-induced organ damage, but their specific function in SCM is unclear.
  • Identifying Neutrophil-Related Genes (NRGs) is crucial for understanding SCM and finding diagnostic/treatment biomarkers.

Purpose of the Study:

  • To investigate the role of Neutrophil-Related Genes (NRGs) in the pathogenesis of septic cardiomyopathy (SCM).
  • To identify potential diagnostic and therapeutic biomarkers for SCM based on NRGs.
  • To elucidate the molecular mechanisms involving neutrophils in SCM.

Main Methods:

  • Differential gene expression analysis of datasets GSE79962 and GSE44363 to identify shared DEGs.
  • Utilized Cytoscape software and the cytoHubba plugin to pinpoint hub DEGs.
  • Integrated hub DEGs with NRGs from WGCNA to identify the Neutrophil-Related Hub Gene (NRHG) MRC1.
  • Validated MRC1 expression in SCM using internal and external datasets.
  • Constructed and validated a neutrophil-related competing endogenous RNA (ceRNA) network (AC145207.5/miR-23a-3p/MRC1).

Main Results:

  • Identified shared differentially expressed genes (DEGs) between sepsis and control groups.
  • Pinpointed MRC1 as a key Neutrophil-Related Hub Gene (NRHG) implicated in SCM.
  • Confirmed abnormal expression of MRC1 in SCM patients through validation studies.
  • Established a functional neutrophil-related ceRNA network involving AC145207.5, miR-23a-3p, and MRC1.

Conclusions:

  • MRC1 is identified as a potential NRHG contributing to SCM pathogenesis.
  • The study provides insights into neutrophil-mediated mechanisms underlying SCM.
  • MRC1 emerges as a promising molecular target for the early diagnosis and therapeutic intervention of SCM.

Keywords:
MRC1biomarkerceRNA networkneutrophils-related geneseptic cardiomyopathy

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