Covalent association between human thymus leukemia-like antigens and CD8(Tp32) molecules
Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1985
Summary
Human thymocytes possess TL-like molecules that associate with CD8 or beta 2 microglobulin, forming distinct CD8-TL complexes and alternative structures. These findings reveal new insights into CD8 complex composition and T cell development.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Thymocytes express several T lymphocyte-like (TL) molecules.
- TL-like molecules are found associated with CD8 (Tp32) and beta 2 microglobulin on human thymocytes.
Purpose of the Study:
- To investigate the molecular associations of TL-like molecules with CD8 and beta 2 microglobulin.
- To characterize the composition of CD8 complexes involving TL-like subunits.
Main Methods:
- Co-precipitation assays to identify associated proteins.
- Analysis of molecular weight complexes using SDS-PAGE under reducing and non-reducing conditions.
Main Results:
- TL-like molecules form the 45 kDa subunit of the CD8 complex.
- CD8-TL complexes are distinct from beta 2 microglobulin associations.
- CD8 exists in various molecular weight complexes, some containing both 32 kDa and 45 kDa (TL-like) subunits.
Conclusions:
- CD8 and beta 2 microglobulin represent alternative association partners for TL-like molecules.
- The composition of CD8 complexes varies, indicating differential assembly or post-translational modification.
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