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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

MicroRNAs implicated in canine diffuse large B-cell lymphoma prognosis.

Nelly O Elshafie¹, Michael Gribskov², Nathanael I Lichti³

¹Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.

|September 1, 2024

Summary

This summary is machine-generated.

Specific microRNAs (miRNAs) correlate with outcomes in dogs with diffuse large B-cell lymphoma (DLBCL). Lower expression of miR-192-5p and miR-16-5p indicates longer progression-free survival in canine DLBCL.

Keywords:

cancer biomarkers canine DLBCL diffuse large B‐cell lymphoma miRNAs multicentric sRNA sequencing

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Area of Science:

Veterinary Oncology
Molecular Biology
Genetics

Background:

Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma (NHL) in dogs, mirroring human disease.
Early treatment initiation is crucial for managing rapid DLBCL progression and achieving remission in dogs.

Purpose of the Study:

To investigate the association between microRNA (miRNA) expression and progression-free survival (PFS) in dogs diagnosed with DLBCL.
To identify specific miRNAs that may serve as predictive biomarkers for DLBCL outcomes in canine patients.

Main Methods:

Analysis of small RNA sequencing (sRNA-seq) data from lymph node samples of 44 dogs with DLBCL.
Quantitative PCR (qPCR) was used to validate and quantify the expression levels of selected miRNAs.

Main Results:

A distinct subset of miRNAs was found to be significantly associated with decreased PFS in dogs with DLBCL.
Downregulation of miR-192-5p and miR-16-5p expression correlated with increased PFS in affected dogs.

Conclusions:

miRNA profiling shows potential for identifying dogs with DLBCL likely to experience poor treatment outcomes.
Identifying specific miRNAs linked to canine DLBCL progression could facilitate personalized treatment strategies.