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Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. [development Of A Novel Male Reproductive Toxicity Assessment Method To Predict Male-mediated Effects On The Next Generation].
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. [development Of A Novel Male Reproductive Toxicity Assessment Method To Predict Male-mediated Effects On The Next Generation].

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[Development of a Novel Male Reproductive Toxicity Assessment Method to Predict Male-mediated Effects on the Next Generation].

Satoshi Yokota1

  • 1Division of Cellular & Molecular Toxicology, Center for Biological Safety & Research, National Institute of Health Sciences.

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|September 1, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

New methods rapidly assess male reproductive toxicity, predicting birth defects from sperm DNA damage. This aids safer drug discovery by evaluating male-mediated developmental toxicity.

Keywords:
developmental toxicitymagnetic resonance imagingpulsed field gel electrophoresisreactive blue 2

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Area of Science:

  • Toxicology
  • Reproductive Biology
  • Drug Development

Background:

  • Male reproductive toxicity is a concern for developmental toxicity, potentially causing paternally transmitted birth defects.
  • Sperm genetic and epigenetic information is vital for fetal development.
  • Assessing male reproductive toxicity is crucial for predicting male-mediated developmental toxicity.

Purpose of the Study:

  • To provide an overview of chemical and male reproductive toxicity.
  • To demonstrate novel methods for evaluating male reproductive toxicity and anticipating male-mediated developmental toxicity.
  • To accelerate drug discovery and repositioning through improved toxicity assessments.

Main Methods:

  • Developed a mammalian male germ cell-specific staining technique using reactive blue 2 dye (RB2).
reproductive toxicity
spermatogenesis
  • Established a method for detecting early-stage DNA fragmentation in mouse spermatozoa using single-cell pulsed-field gel electrophoresis.
  • Utilized a compact magnetic resonance imaging (MRI) platform with a high-field permanent magnet for noninvasive in vivo evaluation of male reproductive toxicity.

    • Main Results:

    • Demonstrated a novel staining technique for evaluating sperm quality and detecting DNA fragmentation.
    • Showcased a compact MRI platform for rapid, noninvasive in vivo assessment of male reproductive toxicity.
    • Histopathological analysis confirmed the suitability of the MRI platform for toxicity evaluation.

    Conclusions:

    • Novel toxicity assessments can predict male-mediated developmental toxicity.
    • The developed methods offer rapid and noninvasive evaluations of male reproductive toxicity.
    • These advancements contribute to accelerated drug discovery and repositioning by enhancing safety evaluations.