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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jun 14, 2025

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Fluorescence-based techniques for investigating estrogen receptor dynamics.

Kiseok Han¹, Gyuho Choi¹, Tae-Jin Kim²

¹Department of Integrated Biological Science, Pusan National University, Busan 46241, Korea.

|September 2, 2024

Summary

Fluorescent probes visualize estrogen receptor (ER) dynamics in living cells, revealing complex genomic and non-genomic signaling pathways. This review details their use in studying ER interactions, transcriptional activation, and GPER signaling for disease research.

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Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Estrogen receptor (ER) signaling is vital for understanding estrogen-related diseases like breast cancer and environmental endocrine disruptors.
ER signaling traditionally involves nuclear genomic events but also includes rapid non-genomic pathways.
Investigating ER dynamics in living cells requires advanced visualization techniques.

Purpose of the Study:

To systematically review the application of fluorescent probes (FPb) in studying estrogen receptor (ER) signaling pathways.
To provide insights into using FPb for visualizing ER dynamics, interactions, and functions in real-time.
To cover various aspects of ER signaling, including genomic and non-genomic pathways and GPER signaling.

Main Methods:

Utilizing advanced fluorescence-based techniques and fluorescent probes (FPb).
Observing ER spatial distribution, expression kinetics, and functional activities in living cells.
Systematic review of literature on FPb applications in ER signaling research.

Main Results:

Fluorescent probes enable visualization of ER localization, ligand-receptor interactions, and receptor dimerization.
FPb facilitate the study of estrogen response elements (EREs)-mediated transcriptional activation.
The review covers the application of FPb in understanding G-protein-coupled estrogen receptor (GPER) signaling.

Conclusions:

Fluorescent probes are powerful tools for dissecting complex ER signaling mechanisms in living cells.
FPb offer valuable insights into both genomic and non-genomic ER functions.
This review equips researchers with knowledge to effectively employ FPb in ER signaling studies.