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Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

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Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
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Abnormal Proliferation02:23

Abnormal Proliferation

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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Fosl1 Promotes Stem Cell‑like Characteristics And Anoikis Resistance To Facilitate Tumorigenesis And Metastasis In Osteosarcoma By Targeting Sox2.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Fosl1 Promotes Stem Cell‑like Characteristics And Anoikis Resistance To Facilitate Tumorigenesis And Metastasis In Osteosarcoma By Targeting Sox2.

Related Experiment Video

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

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FOSL1 promotes stem cell‑like characteristics and anoikis resistance to facilitate tumorigenesis and metastasis in osteosarcoma by targeting SOX2.

Yang Wang1, Qin Hu2, Ya Cao3

  • 1Department of Emergency Medicine Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610064, P.R. China.

International Journal of Molecular Medicine
|September 2, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Fos-like antigen 1 (FOSL1) promotes osteosarcoma metastasis by enhancing cancer stem cell properties and anoikis resistance, primarily by upregulating SOX2 transcription. Targeting FOSL1 may offer new therapeutic strategies for osteosarcoma.

Keywords:
FOSL1OSSOX2anoikis resistance

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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

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Related Experiment Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
09:25

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Published on: October 14, 2016

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Metastasis is a primary cause of cancer mortality in osteosarcoma (OS).
  • Cancer stem cells (CSCs) and anoikis resistance are critical for OS metastasis, but underlying mechanisms remain unclear.
  • Fos-like antigen 1 (FOSL1) is implicated in stemness in other cancers, but its role in OS CSCs and anoikis is unknown.

Purpose of the Study:

  • To investigate the role of FOSL1 in maintaining stem-like properties and anoikis resistance in osteosarcoma.
  • To elucidate the molecular mechanisms by which FOSL1 influences OS stemness and metastasis.
  • To evaluate FOSL1 as a potential therapeutic target for osteosarcoma.

Main Methods:

  • Investigated FOSL1 expression using RNA sequencing, PCR, Western blotting, and immunofluorescence.
stemness
  • Assessed stem cell properties and anoikis resistance via flow cytometry, tumor-sphere, clone formation, and anoikis assays.
  • Utilized in vivo xenograft and metastasis models, and analyzed molecular mechanisms involving SOX2, BCL2, and BAX.

    • Main Results:

    • FOSL1 expression was upregulated in OS stem cells (OSCs) and promoted sphere/clone formation and tumorigenesis.
    • FOSL1 positively correlated with stemness factors (SOX2, NANOG, CD117, Stro1) and enhanced anoikis resistance.
    • FOSL1 promoted metastasis by regulating BCL2/BAX and upregulated SOX2 transcription via promoter interaction.

    Conclusions:

    • FOSL1 drives osteosarcoma stemness and anoikis resistance, facilitating metastasis.
    • FOSL1 exerts its effects by transcriptionally upregulating SOX2.
    • FOSL1 represents a potential therapeutic target for osteosarcoma treatment.