Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

EpCAM<sup>+</sup> Extracellular Vesicle PD-L1 Dynamics as a Predictive Biomarker of Immune Checkpoint Blockade Response.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Premna odorata extract exhibits anti-inflammatory activity via suppression of NLRP3 inflammasome.

Scientific reports·2026

Same author

Enhanced Enzyme Cascade Reactions Through Coencapsulation in Biocompatible Silica Nanoconfinement.

Chembiochem : a European journal of chemical biology·2026

Same author

Biomimetic Compartmentalization of Enzymes for Sustainable Coenzyme Recycling in Oxidative Photocatalysis.

Chembiochem : a European journal of chemical biology·2026

Same author

Polystyrene nanoplastic-encapsulated extracellular vesicles promote blood-brain barrier breakdown and accumulate in the brain.

Journal of hazardous materials·2026

Same author

Electroacupuncture-induced Changes in Motor Protein Pathways in Postpartum Stress Urinary Incontinence.

Journal of visualized experiments : JoVE·2026

Search research articles

Related Experiment Video

Updated: Apr 10, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Microbead-Based Colorimetric and Portable Sensors for Polyphenol Detection.

Suhui Jeong¹, So Young Kim¹, Hwain Myeong¹

¹Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea.

|September 2, 2024

Summary

New portable beads enable rapid, on-site colorimetric quantification of total polyphenols in beverages. This method offers a simple, 5-minute detection process with improved sensitivity compared to traditional assays.

More Related Videos

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Published on: June 9, 2014

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Related Experiment Videos

Last Updated: Apr 10, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Published on: June 9, 2014

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Area of Science:

Analytical Chemistry
Materials Science

Background:

Polyphenols in health supplements and beverages possess antioxidant and anti-inflammatory properties.
Accurate, on-site quantification of total polyphenols is crucial for assessing the beneficial qualities of these products.
Existing methods like the Folin-Ciocalteu assay require laboratory settings and are less accessible for field detection.

Purpose of the Study:

To develop a portable, colorimetric method for the on-site quantification of total polyphenols.
To create a user-friendly detection system utilizing smartphone technology.
To evaluate the performance of the developed bead-based sensor against established methods.

Main Methods:

Development of portable beads containing ferric ions that react with polyphenols to form colored complexes.
A simple detection procedure involving dipping beads in a sample, taking a photo with a smartphone or tablet, and converting color values for quantification.
Comparison of the bead-based sensor's limit of detection and sensing capability with the Folin-Ciocalteu assay using tannic acid and polyphenol-containing samples.

Main Results:

The developed bead-based sensor provides colorimetric quantification of polyphenols within 5 minutes.
The sensor demonstrated a lower limit of detection (0.0415 mM for tannic acid) compared to the Folin-Ciocalteu assay.
The beads maintained their shape and sensitivity after prolonged storage and under varying environmental conditions (temperature).

Conclusions:

The portable bead-based sensor offers a simple, rapid, and sensitive method for on-site total polyphenol quantification.
This technology is suitable for field detection of polyphenols in beverages and health supplements.
The developed sensor shows promise as a cost-effective and accessible alternative to traditional laboratory-based assays.