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Suppressor mutations causing partial reversion in the amiA region of Pneumococcus.

Y George, L O Butler

    Molecular & General Genetics : MGG
    |July 24, 1979
    PubMed
    Summary
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    New suppressor genes in pneumococcus mutants were identified. These genes reduced isoleucine sensitivity without affecting aminopterin resistance, dissociating linked traits.

    Area of Science:

    • Microbiology
    • Genetics
    • Bacterial Resistance

    Background:

    • Pneumococcus mutants resistant to aminopterin also exhibit sensitivity to branched-chain amino acids.
    • This linked phenotype is associated with mutations in the amiA cistron.
    • Understanding the genetic basis of these linked traits is crucial for bacterial resistance studies.

    Purpose of the Study:

    • To isolate and characterize suppressor mutations that can dissociate aminopterin resistance from isoleucine sensitivity in pneumococcus.
    • To investigate the effect of these suppressor genes on other resistance mechanisms.
    • To map the location of these novel suppressor mutations within the pneumococcus genome.

    Main Methods:

    • Mutagenesis of an aminopterin-resistant pneumococcus strain using 5-BUdR.

    Related Experiment Videos

  • Phenotypic analysis of suppressed strains for aminopterin resistance and branched-chain amino acid sensitivity.
  • Recombination and clonal analysis for mapping suppressor gene loci.
  • Testing the effect of suppressor genes on resistance to other antibacterial substances.

    • Main Results:

    • Four distinct suppressor genes were identified in 5-BUdR-mutagenized pneumococcus.
    • These suppressor mutations partially reversed the isoleucine sensitivity phenotype while maintaining aminopterin resistance.
    • The suppressor genes specifically affected isoleucine sensitivity linked to amiA cistron mutations and did not impact resistance to other agents.
    • Mapping indicated that the suppressor mutations are intragenic and located near known amiA loci.

    Conclusions:

    • Suppressor gene mutations can functionally dissociate linked phenotypes, such as drug resistance and amino acid sensitivity, in bacteria.
    • These findings provide new insights into the genetic regulation of bacterial resistance mechanisms in Streptococcus pneumoniae.
    • The identified suppressor genes offer potential tools for further genetic and biochemical studies of the amiA cistron and related pathways.