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Related Experiment Video

Updated: Jun 14, 2025

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

Rapid Tissue Perfusion Using Sacrificial Percolation of Anisotropic Networks.

Alex Lammers^1,2, Heng-Hua Hsu³, Subramanian Sundaram^1,2

¹The Biological Design Center and Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

|September 2, 2024

Summary

This summary is machine-generated.

A new technique, Sacrificial Percolation of Anisotropic Networks (SPAN), rapidly creates perfusable vascular networks in engineered tissues. This method overcomes limitations of current tissue engineering approaches for scalable vascularization.

Keywords:

anisotropic percolation biofabrication microfluidics sacrificial casting self-assembly tissue engineering vascular engineering vessel network volumetric subtractive manufacturing

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Last Updated: Jun 14, 2025

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

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Published on: April 4, 2018

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Published on: August 13, 2020

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Published on: July 20, 2022

Area of Science:

Biomaterials Science
Regenerative Medicine
Vascular Tissue Engineering

Background:

Current tissue engineering methods struggle to rapidly create perfusable vascular networks across diverse vessel sizes.
Existing top-down and bottom-up techniques face limitations in scalability and the resolution-assembly time tradeoff.

Purpose of the Study:

To introduce a novel, flexible, and scalable technique for rapid vascular network generation in engineered tissues.
To overcome the limitations of existing methods in achieving rapid vascularization irrespective of construct size.

Main Methods:

Development of Sacrificial Percolation of Anisotropic Networks (SPAN), a technique utilizing pipettable alginate fibers.
Interconnection of alginate fibers above a percolation density threshold to form a network.
Degradation of sacrificial alginate fibers to create perfusable channels within constructs of arbitrary size and shape.

Main Results:

SPAN generates perfusable channel networks spanning arteriole to capillary scales within minutes.
The technique is size-independent, applicable to constructs of arbitrary dimensions.
SPAN facilitates the generation of endothelial cell-lined vasculature within multi-cell type constructs.

Conclusions:

SPAN offers a rapid and scalable solution for creating perfusable vascular networks in engineered tissues.
This technique integrates seamlessly with existing tissue engineering processes.
SPAN holds significant potential for advancing the rapid assembly of complex, vascularized tissues.