Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.6K
Development of Blood Vessels01:07

Development of Blood Vessels

549
The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
549

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exercise Resistance in Obese Male NZO Mice Manifests as Local Muscle Remodelling Without Glycaemic Improvements.

Diabetes, obesity & metabolism·2026
Same author

Controlling 3D Contractility via Engineered Fibrous Hydrogel Composites.

Advanced functional materials·2026
Same author

The dorsal aortic compartment is a developmental source of brown adipose tissue in mice.

Nature communications·2026
Same author

Definition, Epidemiology and Pathophysiology of Lymphoedema.

Cells·2025
Same author

Insights into KIF11 pathogenesis in microcephaly-lymphedema-chorioretinopathy syndrome from a lymphatic perspective.

JCI insight·2025
Same author

Modeling immune lineage co-development in human pluripotent stem cell-derived liver organoids.

Journal of hepatology·2025

Related Experiment Video

Updated: Jun 14, 2025

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

14.3K

Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation.

Pedro Vallecillo-García1,2, Mira Nicola Kühnlein1, Mickael Orgeur3

  • 1Institute for Chemistry and Biochemistry, Freie Universität Berlin,14195 Berlin, Germany.

Development (Cambridge, England)
|September 2, 2024
PubMed
Summary

Mesenchymal Osr1+ cells guide lymphatic vessel development by controlling lymphatic endothelial cell (LEC) migration and proliferation. These cells produce essential extracellular matrix components and signaling ligands for lymphatic system formation.

Keywords:
Cxcl12Extracellular matrixLymphatic vasculatureMesenchymal cellOsr1

More Related Videos

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

17.6K
Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids
07:37

Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids

Published on: August 16, 2024

1.2K

Related Experiment Videos

Last Updated: Jun 14, 2025

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

14.3K
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

17.6K
Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids
07:37

Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids

Published on: August 16, 2024

1.2K

Area of Science:

  • Developmental biology
  • Vascular biology
  • Cell biology

Background:

  • The lymphatic system is crucial for fluid homeostasis and immunity.
  • Lymphatic endothelial cells (LECs) form lymphatic vessels during embryonic development.
  • The role of associated mesenchymal cells in lymphatic development is largely unknown.

Purpose of the Study:

  • To investigate the role of mesenchymal cells in lymphatic vasculature development.
  • To identify specific mesenchymal cell populations interacting with LECs.
  • To elucidate the mechanisms by which mesenchymal cells influence LEC behavior.

Main Methods:

  • Lineage tracing in mouse embryos.
  • Analysis of Osr1-deficient embryos.
  • In vitro functional assays.
  • Immunohistochemistry and microscopy.

Main Results:

  • A subpopulation of mesenchymal cells expressing Osr1 (Osr1+ cells) are closely associated with LECs.
  • Osr1+ cells precede LECs during lymphatic vessel assembly in the embryo.
  • Osr1 acts non-cell-autonomously to control LEC proliferation and migration.
  • Mesenchymal Osr1+ cells regulate extracellular matrix and signaling ligands essential for lymphatic formation.

Conclusions:

  • Mesenchymal Osr1+ cells play a critical, bimodal role in lymphatic vasculature development.
  • Osr1+ cells are essential regulators of LEC behavior and lymphatic patterning.
  • This study reveals a novel mesenchymal contribution to lymphatic system formation.