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Arabinoxylan from pearl millet bran: Optimized extraction, structural characterization, and its bioactivities
Akanksha Singh1, Deependra Rajoriya2, Indudhar S Obalesh3
1Department of Traditional Foods and Applied Nutrition, CSIR-Central Food Technological Research Institute, Mysore 570020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
International Journal of Biological Macromolecules
|September 2, 2024
Summary
Pearl millet bran yields arabinoxylan (PMAX) with potent antioxidant and anti-glycation properties. This PMAX also supports beneficial gut bacteria, suggesting potential nutraceutical applications.
Area of Science:
- Food Science and Technology
- Nutraceuticals
- Biochemistry
Background:
- Cereal and millet arabinoxylans (AX) are recognized for their bioactivities.
- Pearl millet bran is an underutilized source of valuable compounds.
- Arabinoxylan's potential in functional foods and health applications is gaining interest.
Purpose of the Study:
- To optimize the extraction of arabinoxylan (PMAX) from pearl millet bran.
- To characterize the structural and physicochemical properties of PMAX.
- To evaluate the bioactivities of PMAX, including antioxidant, anti-glycation, and gut microbiota modulation.
Main Methods:
- Optimized alkali-assisted extraction using Response Surface Methodology and Central Composite Design.
- Structural analysis using molar composition, linkage analysis, and molecular weight determination.
- In vitro assays for reducing power, metal chelating activity, radical scavenging, anti-glycation, and protein oxidation inhibition.
- In vitro and in vivo (male Wistar rats) studies on gut microbiota modulation.
Main Results:
- Achieved a high yield (15.96%) of PMAX under optimized conditions.
- PMAX characterized by a specific sugar composition, highly substituted backbone, and high molecular weight (794.88 kDa).
- Demonstrated significant antioxidant (reducing power, radical scavenging) and anti-glycation activities.
- PMAX promoted Lactobacillus growth and modulated gut microbiota in rats (increased Bacteroidetes, decreased Firmicutes).
Conclusions:
- Pearl millet arabinoxylan (PMAX) is efficiently extracted and possesses significant antioxidant and anti-glycation properties.
- PMAX exhibits potential as a prebiotic and gut microbiota modulator.
- These findings support the exploration of PMAX for nutraceutical and functional food applications.
