Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

ACS chemical biology·2026
Same author

Advanced CD276-Targeting Dual-Payload Antibody-Drug Conjugates for Cancer Therapy.

Cancer research communications·2026
Same author

Discovery of a Minimally Charged Cell-Penetrating Peptide.

Biochemistry·2026
Same author

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

bioRxiv : the preprint server for biology·2026
Same author

Endosomal Escape of Lipid Nanoparticles: A Perspective on the Literature Data.

ACS nano·2025
Same author

Anti-lipolysis-stimulated lipoprotein receptor antibody-drug conjugate to treat triple-negative breast cancer.

Frontiers in oncology·2025
Same journal

Rescuing Dendritic Cells from Adjuvant Toxicity: Liposomal Ginsenoside Rh2 as a Dual-Action Strategy for Enhanced Vaccine Potency.

Molecular pharmaceutics·2026
Same journal

First-in-Class CD146-Targeting Peptide Probes for Noninvasive PET Imaging of Melanoma.

Molecular pharmaceutics·2026
Same journal

Dual-Targeted Radionuclide Therapy with <sup>161</sup>Tb Instigates Anticancer Immunity in "Cold" Murine Prostate Tumor.

Molecular pharmaceutics·2026
Same journal

Development of [<sup>111</sup>In]In-CHX-A″-DTPA-αCD68 for ImmunoSPECT to Image Murine Macrophages.

Molecular pharmaceutics·2026
Same journal

Mechanistic Insight into Self-Gelation Involved in Prescription Design for Optimization of Tablet Performance.

Molecular pharmaceutics·2026
Same journal

[<sup>68</sup>Ga]Ga-DOTA-DP-UBI 29-41: A Novel <sup>68</sup>Ga-Labeled Ubiquicidin 29-41 Derivative Containing d-Proline for Bacterial Infection PET Imaging.

Molecular pharmaceutics·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.4K

Bismuth-Cyclized Cell-Penetrating Peptides.

Jeremy L Ritchey1, Lindsi Filippi1, Davis Ballard1

  • 1Department of Chemistry and Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio 43210, United States.

Molecular Pharmaceutics
|September 3, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed novel bismuth cyclized cell-penetrating peptides (CPPs) for efficient intracellular delivery. These CPPs offer improved cytosolic entry, stability, and potential for recombinant production, advancing research tools and therapeutics.

Keywords:
bismuthcell-penetrating peptidesdrug deliveryendosomal escapepeptide cyclization

More Related Videos

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.3K
Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.0K

Related Experiment Videos

Last Updated: Jun 18, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.4K
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.3K
Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Delivery

Background:

  • Intracellular delivery of biological cargos is crucial for developing new research tools and therapeutics.
  • Cell-penetrating peptides (CPPs) are a promising strategy for cargo delivery, but linear CPPs have limitations.
  • Existing backbone-cyclized CPPs require chemical synthesis and posttranslational modification, and can undergo epimerization.

Purpose of the Study:

  • To develop a new class of cell-penetrating peptides (CPPs) with enhanced properties for intracellular delivery.
  • To overcome the limitations of linear and previously developed cyclized CPPs.
  • To explore the potential for genetic encoding and recombinant production of these novel CPPs.

Main Methods:

  • Design and synthesis of a novel class of bismuth-cyclized CPPs.
  • Evaluation of cytosolic entry efficiency.
  • Assessment of proteolytic stability.
  • Exploration of compatibility with genetic encoding and recombinant production.

Main Results:

  • The novel bismuth-cyclized CPPs demonstrated excellent cytosolic entry efficiencies.
  • These CPPs exhibited significant proteolytic stability compared to existing methods.
  • The developed CPPs show potential for integration with genetic encoding and recombinant production systems.

Conclusions:

  • Bismuth-cyclized CPPs represent a promising new class of peptides for efficient intracellular cargo delivery.
  • This approach overcomes key limitations of previous CPP strategies, including chemical synthesis and stability issues.
  • The compatibility with recombinant production opens avenues for scalable and cost-effective therapeutic development.