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Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

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Serotonin's Role in Inflammatory Signaling Pathway Modulation for Colon Cancer Suppression.

Supreeta Maheshwarla Saravanan¹, Lavanya Prathap¹, Jabir Padathpeedika Khalid²

¹Department of Anatomy, Biomedical Research Unit and Laboratory Animal Centre (BRULAC) Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.

|September 3, 2024

Summary

Serotonin effectively inhibits growth and downregulates key inflammatory and cancer markers in KB cells. This study highlights serotonin

Keywords:

adjuvant chemotherapy anti-inflammatory activity anti-inflammatory effect cancer inhibition colon cancer intermediate risk mismatch repair serotonin

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Area of Science:

Oncology
Neuroscience
Molecular Biology

Background:

Serotonin and dopamine regulate neurons, with potential in anti-inflammatory and chemotherapeutic research.
The study focuses on serotonin's anti-inflammatory and anticancer effects in HeLa (KB) cells.

Purpose of the Study:

Investigate serotonin's role in suppressing inflammation and its anticancer potential in KB cells.
Analyze molecular mechanisms of serotonin's action, including its interaction with Interleukin-6.

Main Methods:

In vitro and in silico analysis of serotonin's effects on KB cells.
Real-time RT-PCR to assess B-cell leukemia/lymphoma 2 protein (BCl-2), tumor necrosis factor-alpha (TNF-α), and Interleukin-6 (IL-6) expression.
Molecular docking using Schrodinger suites to analyze serotonin binding with human Interleukin-6.

Main Results:

Serotonin demonstrated significant growth inhibition in KB cells (IC50: 225±3.1µg/ml).
Downregulation of BCl-2, TNF-α, and IL-6 expression observed.
Molecular docking revealed efficient binding of serotonin to human Interleukin-6 (docking score: -5.65 kcal/mol).
Serotonin blocked NF-κB pathways in KB cells.

Conclusions:

Serotonin exhibits significant anticancer potential by blocking NF-κB pathways in KB cells.
Findings suggest serotonin as a promising therapeutic agent for colon cancer.
The study provides insights into serotonin's molecular interactions for potential clinical applications.