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Updated: Jun 14, 2025
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
Published on: June 2, 2020
Extracellular vesicles released by Trypanosoma evansi: induction analysis and proteomics.
Amanda Martins Ungri1, Bruna Fernanda Dos Santos Sabatke2, Izadora Volpato Rossi2
1Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil.
Extracellular vesicles (EVs) from Trypanosoma evansi, the parasite causing surra disease, were analyzed. While calcium did not boost EV production, proteomic analysis identified unique T. evansi epitopes as potential biomarkers for surra.
Area of Science:
- Parasitology
- Molecular Biology
- Biochemistry
Background:
- Trypanosoma evansi causes the economically significant disease surra in livestock.
- Extracellular vesicles (EVs) are increasingly recognized for their role in intercellular communication and as potential disease biomarkers.
- Limited knowledge exists regarding T. evansi virulence factors and secreted EVs.
Purpose of the Study:
- To investigate the effect of calcium (Ca+2) on T. evansi EV production.
- To perform proteomic analysis of T. evansi EVs.
- To identify potential biomarker epitopes within T. evansi EVs.
Main Methods:
- T. evansi cultures were treated with Ca+2 to assess vesiculation.
- Proteomic analysis using mass spectrometry was performed on isolated EVs.
- Bioinformatic tools were used to identify unique T. evansi proteins and epitopes.
Main Results:
- Calcium (Ca+2) was found to be ineffective in inducing EV production in T. evansi.
- Proteomic analysis identified 442 proteins within the EVs.
- Seven proteins recognized nine unique T. evansi epitopes, with one previously identified epitope (TevSTIB805.9.11580) showing promise.
Conclusions:
- EVs from T. evansi contain numerous proteins, some with potential as biomarkers or vaccine targets.
- The identified unique epitopes warrant further investigation for diagnostic and therapeutic applications in surra.
- T. evansi EVs represent a valuable source for discovering biomarkers for surra detection.
