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J-chain expression in human cells producing IgG subclasses.

P L Haber, J Mestecky

    Cellular Immunology
    |April 1, 1985
    PubMed
    Summary
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    J chain is found in cells producing monomeric IgG, not just polymeric immunoglobulins. Its expression varies across IgG subclasses and may relate to cell maturation and origin.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • J chain is known to be essential for polymeric immunoglobulin production.
    • Previous findings suggest J chain's role extends beyond polymer formation, appearing in monomeric immunoglobulin-producing cells like IgG and IgD.

    Purpose of the Study:

    • To investigate the distribution of J chain in immunoglobulin G (IgG) plasma cells.
    • To determine if J chain expression differs among cells producing various IgG subclasses.

    Main Methods:

    • Utilized fluorochrome-labeled polyclonal anti-J-chain antibodies.
    • Employed monoclonal antibodies specific to IgG subclasses.
    • Analyzed J chain distribution in human lymphoid tissues and pokeweed mitogen (PWM)-stimulated peripheral blood lymphocytes.

    Main Results:

    Related Experiment Videos

    • J chain expression was not uniform across IgG subclasses in PWM-stimulated cells (IgG1: 22%, IgG2: 49%, IgG3: 17%, IgG4: 64%).
    • In human lymphoid tissues, J chain coexpression in IgG cells was less frequent than in the PWM system and varied by IgG subclass.
    • Observed differences in J-chain frequency among IgG-producing cells.

    Conclusions:

    • J chain's presence in IgG-producing cells indicates a role beyond polymeric immunoglobulin synthesis.
    • The variable distribution of J chain among IgG subclasses suggests subclass-specific regulation.
    • J-chain expression in IgG cells may correlate with cellular maturation and cell origin.