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Positive and negative reinforcement are key concepts in operant conditioning, a learning process where the consequences of a behavior affect the likelihood of that behavior being repeated.
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Related Experiment Video

Updated: Jun 14, 2025

Negative Additive Manufacturing of Complex Shaped Boron Carbides
06:45

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Turning a negative into a positive.

John F Foley1

  • 1Science Signaling, AAAS, Washington, DC 20005, USA.

Science Signaling
|September 3, 2024
PubMed
Summary
This summary is machine-generated.

Negatively charged lipid bilayers significantly boost interactions between chemokines and atypical chemokine receptors. This finding advances our understanding of chemokine receptor binding dynamics.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Chemokines are crucial signaling proteins involved in immune cell trafficking.
  • Atypical chemokine receptors (ACKRs) modulate chemokine availability and signaling.
  • The biophysical properties of cell membranes can influence receptor-ligand interactions.

Purpose of the Study:

  • To investigate the impact of lipid bilayer charge on chemokine-atypical chemokine receptor interactions.
  • To elucidate the role of negatively charged membranes in modulating ACKR binding affinity.

Main Methods:

  • Utilized surface plasmon resonance (SPR) to quantify binding kinetics.
  • Employed liposome-based assays with varying lipid compositions to mimic different membrane charges.
  • Measured the binding affinity of representative chemokines to ACKRs reconstituted in these lipid bilayers.

Main Results:

  • Negatively charged lipid bilayers significantly enhanced the binding affinity of chemokines to atypical chemokine receptors.
  • The magnitude of enhancement correlated with the density of negative charges on the lipid bilayer.
  • This effect was specific to the interaction between chemokines and ACKRs, not observed with typical chemokine receptors.

Conclusions:

  • The negative charge of lipid bilayers plays a critical role in promoting chemokine binding to atypical chemokine receptors.
  • This electrostatic interaction mechanism provides a new perspective on regulating chemokine gradients and immune responses.
  • Findings suggest membrane charge as a potential target for modulating ACKR activity in therapeutic strategies.